Overview

  • Product name
    Anti-GST antibody [3D4]
    See all GST primary antibodies
  • Description
    Mouse monoclonal [3D4] to GST
  • Specificity
    This antibody reacts specifically with GST.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Schistosoma japonicum (common blood fluke). Not yet tested in other species.
  • Immunogen

    Glutathione S Transferase (Schistosoma japonicum).

  • Positive control
    • WB: HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab34589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000 - 1/100000.
WB 1/1000 - 1/10000. Detects a band of approximately 26 kDa.

Target

  • Relevance
    GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
  • Alternative names
    • Glutathione S Transferase antibody
    • Glutathione S-transferase class-mu 26 kDa isozyme antibody
    • GST antibody
    • Sj26 antigen antibody
    • SjGST antibody
    see all

Images

  • Western blot using mouse monoclonal [3D4] anti-GST antibody (ab34589) to detect purified GST recombinant protein (left) and in a lysate of HeLa cells expressing recombinant GST protein (right). Approximately 0.1µg of protein was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with primary antibody diluted to 1.0µg/ml for 1 h at room temperature, followed by washes and reaction with a 1:5000 dilution of IRDye 800 conjugated Gt-a-Mouse IgG [H&L] MX. The fluorescence image was captured using a LI-COR imaging system. Band size: approximately 26kDa.

References

This product has been referenced in:
  • Nakashima H  et al. Interferon-stimulated gene 15 (ISG15) and ISG15-linked proteins can associate with members of the selective autophagic process, histone deacetylase 6 (HDAC6) and SQSTM1/p62. J Biol Chem 290:1485-95 (2015). Read more (PubMed: 25429107) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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