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Full length protein corresponding to Schistosoma japonicum GST aa 2-218.
SPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLE FPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLD IRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHP DFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAW PLQGWQATFGGGDHPPK
Our Abpromise guarantee covers the use of ab181652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000.|
|ELISA||1/200 - 1/1000.
ab181652 has been assayed against 1.0 ug of Glutathione-S-Transferase [Schistosoma japonicum] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid) as a substrate for 30 minutes at room temperature. A working dilution of 1/3,000 to 1/15,000 of the reconstitution concentration is suggested for this product.
|IHC-P||Use at an assay dependent concentration.|
ab181652 was used to detect GST under reducing and non-reducing conditions. Reduced samples of purified GST contained 4% BME and were boiled for 5 minutes. For lanes 1-4, samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with a Goat anti GST at 1/5000 dilution overnight at 4°C. Primary antibody was detected with a conjugated Donkey anti Goat at 1/10000, incubated for 1.5 hr at RT and imaged on the BioRad VersaDoc imaging system. Lanes 5-6 show a repeat western blot with the same samples (~1 ug per lane, reduced (R) and nonreduced (NR) detected with a Dylight 549 conjugated Donkey anti goat incubated at 1/10000 at 1.5 hours at RT.
ab181652 has not yet been referenced specifically in any publications.