The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use 15-25 µl of gel slurry per 0.1 to 1 mg of protein lysate or extract.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceGST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.