Whole antisera is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins. The IgG fraction is then conjugated to calf intestinal alkaline phosphatase using glutaraldehyde.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 when using BCIP/NBT as substrate, 1/20,000 when using a chemiluminescent
substrate. Detects a band of approximately 27.5 kDa (predicted molecular weight: 26 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.