Overview

  • Product nameAnti-GST antibody [EPR4236]
    See all GST primary antibodies
  • Description
    Rabbit monoclonal [EPR4236] to GST
  • Tested applicationsSuitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (GST-tag) corresponding to GST aa 300-450. GST tag was fused to ATG16L1 to produce a recombinant protein.
    Database link: Q676U5

  • Positive control
    • GST fusion proteins; Alpha-actinin-4-GST, AKT1/PKBa-GST or CDK7-GST.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab111947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
ICC/IF 1/100.
IP 1/20.
  • Application notesIs unsuitable for Flow Cyt or IHC-P.
  • Target

    • RelevanceGST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
    • Alternative names
      • Glutathione S Transferase antibody
      • Glutathione S-transferase class-mu 26 kDa isozyme antibody
      • GST antibody
      • Sj26 antigen antibody
      • SjGST antibody
      see all

    Anti-GST antibody [EPR4236] images

    • Immunofluorescence staining of 293T cells transfected with GST, with ab111947 at a working dilution of 1/100 and counter-stained with DAPI. Tubulin was also stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120). The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1/500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X-100. The negative controls are shown in the bottom hand panels - for the first negative control, anti-GST antibody (ab111947) was used at a dilution of 1/100 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/1000 and for the second negative control, mouse anti-tubulin (ab7291) and anti-rabbit secondary antibody (ab150077) were used.

    • GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.

      Lane 1: Primary: ab111947, 1/1000 dilution
      Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
      Lane 2: Primary: ab111947, 1/1000 dilution
      Sample: 293T transfected with GST tagged protein vector cell lysate
      Lane 3: Primary: ab172730, Rabbit monoclonal IgG
      Sample: 293T transfected with GST tagged protein vector cell lysate

      All lanes: Secondary: ab131366, Veriblot, 1/10000 dilution
      Blocking and diluting buffer: 5% NFDM/TBST

    • Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution + GST recombinant protein at 0.01 µg

      Predicted band size : 26 kDa
    • (Cross IP) GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.

      Lane 1: Primary: ab180123, 1/1000 dilution
      Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
      Lane 2: Primary: ab180123, 1/1000 dilution
      Sample: 293T transfected with GST tagged protein vector cell lysate
      Secondary: ab131366, Veriblot, 1/10000 dilution
      Lane 3: Primary: ab172730, Rabbit monoclonal IgG
      Sample: 293T transfected with GST tagged protein vector cell lysate

      All lanes: Secondary: ab131366, Veriblot, 1/10000 dilution
      Blocking and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution

      Lane 1 : Alpha-actinin-4-GST fusion protein
      Lane 2 : AKT1/PKBa-GST fusion protein
      Lane 3 : CDK7-GST fusion protein

      Lysates/proteins at 0.01 µg per lane.


      Predicted band size : 26 kDa

    References for Anti-GST antibody [EPR4236] (ab111947)

    ab111947 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"