Overview

  • Product name
    Anti-GST antibody [EPR4236]
    See all GST primary antibodies
  • Description
    Rabbit monoclonal [EPR4236] to GST
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (GST-tag) corresponding to GST aa 300-450. GST tag was fused to ATG16L1 to produce a recombinant protein.
    Database link: Q676U5

  • Positive control
    • GST fusion proteins; Alpha-actinin-4-GST, AKT1/PKBa-GST or CDK7-GST.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab111947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
ICC/IF 1/100.
IP 1/20.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Relevance
      GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
    • Alternative names
      • Glutathione S Transferase antibody
      • Glutathione S-transferase class-mu 26 kDa isozyme antibody
      • GST antibody
      • Sj26 antigen antibody
      • SjGST antibody
      see all

    Images

    • Immunofluorescence staining of 293T cells transfected with GST, with ab111947 at a working dilution of 1/100 and counter-stained with DAPI. Tubulin was also stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120). The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1/500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X-100. The negative controls are shown in the bottom hand panels - for the first negative control, anti-GST antibody (ab111947) was used at a dilution of 1/100 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/1000 and for the second negative control, mouse anti-tubulin (ab7291) and anti-rabbit secondary antibody (ab150077) were used.

    • GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.

      Lane 1: Primary: ab111947, 1/1000 dilution
      Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
      Lane 2: Primary: ab111947, 1/1000 dilution
      Sample: 293T transfected with GST tagged protein vector cell lysate
      Lane 3: Primary: ab172730, Rabbit monoclonal IgG
      Sample: 293T transfected with GST tagged protein vector cell lysate

      All lanes: Secondary: ab131366, Veriblot, 1/10000 dilution
      Blocking and diluting buffer: 5% NFDM/TBST

    • Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution + GST recombinant protein at 0.01 µg

      Predicted band size : 26 kDa
    • (Cross IP) GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.

      Lane 1: Primary: ab180123, 1/1000 dilution
      Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
      Lane 2: Primary: ab180123, 1/1000 dilution
      Sample: 293T transfected with GST tagged protein vector cell lysate
      Secondary: ab131366, Veriblot, 1/10000 dilution
      Lane 3: Primary: ab172730, Rabbit monoclonal IgG
      Sample: 293T transfected with GST tagged protein vector cell lysate

      All lanes: Secondary: ab131366, Veriblot, 1/10000 dilution
      Blocking and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution

      Lane 1 : Alpha-actinin-4-GST fusion protein
      Lane 2 : AKT1/PKBa-GST fusion protein
      Lane 3 : CDK7-GST fusion protein

      Lysates/proteins at 0.01 µg per lane.


      Predicted band size : 26 kDa

    References

    This product has been referenced in:

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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