Anti-GST antibody [GST.B6] (ab18183)

Overview

  • Product nameAnti-GST antibody [GST.B6]
    See all GST primary antibodies
  • Description
    Mouse monoclonal [GST.B6] to GST
  • SpecificityDetects overexpressed glutathione-transferase (GST) fusion proteins. Recognizes native and denatured forms of purified GST or GST fusion proteins
  • Tested applicationsSuitable for: ELISA, Dot blot, WB, IP, ICC, ICC/IFmore details
  • Immunogen

    GST fusion protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab18183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.
WB 1/1000 - 1/3000. Dilutions recommended for ECL incubation at Room Temerature for 1 hour.
IP Use at an assay dependent dilution.
ICC 1/500 - 1/2000.
ICC/IF Use at an assay dependent dilution.

Target

  • RelevanceGST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
  • Alternative names
    • Glutathione S Transferase antibody
    • Glutathione S-transferase class-mu 26 kDa isozyme antibody
    • GST antibody
    • Sj26 antigen antibody
    • SjGST antibody
    see all

Anti-GST antibody [GST.B6] images

  • 1:5000 (0.2µg/mL) Ab dilution probed against BL-21 bacteria lysate expressing the GST protein.

References for Anti-GST antibody [GST.B6] (ab18183)

ab18183 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for contacting us and letting us know about the trouble with this antibody.

I'm sorry to hear about the trouble with ab18183. We haven't received other recent reports of problems with the antibody, but we do fully guarante...

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Unfortunately we do not have the kinetic data for this product.

Thank you for contacting us.

While it is likely that this product will work in IF, we have not yet tested ab6613 in this application and therefore cannot guarantee that it will give the desired results in this application. We do have a num...

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Thank you for your enquiry. Mouse monoclonal [GST.B6] to GST (ab18183) can detect 10-50ng recombinant protein based on our in-house data. The antibody is provided in 1X PBS, plus 0.1% Sodium Azide. No BSA was added. I hope this information h...

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Thank you for your enquiry. This antibody was generated using the pGEX GST fusion construct. The clone specific to GST was identified but the epitope mapping was not done. So we are not sure exactly what GST epitope our GST antibody recognizes. I...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"