Overview

  • Product name
    Anti-GST antibody (HRP)
    See all GST primary antibodies
  • Description
    Rabbit polyclonal to GST (HRP)
  • Conjugation
    HRP
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Rabbits were immunized with Glutathione-S-transferase (GST) from Schistosoma japonicum.

Properties

Applications

Our Abpromise guarantee covers the use of ab3416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000.
ELISA 1/10000 - 1/25000.

Target

  • Relevance
    GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
  • Alternative names
    • Glutathione S Transferase antibody
    • Glutathione S-transferase class-mu 26 kDa isozyme antibody
    • GST antibody
    • Sj26 antigen antibody
    • SjGST antibody
    see all

Anti-GST antibody (HRP) images

  • Graph of ELISA measuring levels of bound GST-tagged mosquito proteins. 5g of target protein was coated onto an ELISA plate and incubated overnight at 4°C. The plate was washed and GST-tagged mosquito proteins were incubated for an hour at RT. The proteins were washed off, GST-HRP antibody (ab3416) was diluted 1/5000 in 1%BSA in PBS and added for 30 min at RT. The plate was washed and TMB substrate was added for 20 min at RT. Stop solution was added and the O.D. of the wells read at 450 nm.

    See Abreview

  • Graph of ELISA measuring levels of bound GST-tagged mosquito proteins. 5g of target protein was coated onto an ELISA plate and incubated overnight at 4°C. The plate was washed and GST-tagged mosquito proteins were incubated for an hour at RT. The proteins were washed off, GST-HRP antibody (ab3416) was diluted 1/5000 in 1%BSA in PBS and added for 30 min at RT. The plate was washed and TMB substrate was added for 20 min at RT. Stop solution was added and the O.D. of the wells read at 450 nm.

    See Abreview

References for Anti-GST antibody (HRP) (ab3416)

This product has been referenced in:
  • Karlsson T  et al. A polybasic motif in ErbB3-binding protein 1 (EBP1) has key functions in nucleolar localization and polyphosphoinositide interaction. Biochem J 473:2033-47 (2016). Read more (PubMed: 27118868) »
  • Whalley HJ  et al. Cdk1 phosphorylates the Rac activator Tiam1 to activate centrosomal Pak and promote mitotic spindle formation. Nat Commun 6:7437 (2015). WB . Read more (PubMed: 26078008) »

See all 15 Publications for this product

Product Wall

Abreviews
Application
ELISA
Sample
Anopheles stephensi Recombinant protein (GST-tagged mosquito protein made in e coli)
Specification
GST-tagged mosquito protein made in e coli
Type
Direct
Username

Abcam user community

Verified customer

Submitted Dec 08 2014

Application
ELISA
Sample
Aedes aegypti (Mosquito) Recombinant protein (GST-tagged mosquito protein made in e coli)
Specification
GST-tagged mosquito protein made in e coli
Type
Direct
Username

Abcam user community

Verified customer

Submitted Dec 08 2014

Application
Western blot
Loading amount
1 µg
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Sample
Aedes aegypti (Mosquito) Recombinant protein (Aedes salivary protein)
Specification
Aedes salivary protein
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 26 2014

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

Read More

Thank very much for your patience as it took us more than a month to test this antibody.

The collaborator laboratory have confirmed that they have immunized rabbits with GST isolated from Schistosoma japonicum. The following is their respons...

Read More

Thank you for your email.

Laboratory has agreed to retest this antibody. I will get back to with more details soon.

Many thanks for having patience!

Thank you for contacting us.

The immunogen sequence does not contain thrombin, it is a GST isolated from Schistosoma japonicum species. The antibody should not be binding to His conjugated proteins.

The band in His-thrombin might be a...

Read More

Thank you for your enquiry. I am pleased to hear the ab3416 antibody has worked well in your experiments.

I can confirm that ab3416 and ab106222 are different antibodies.

I would like to reassure you that our antibodies are covere...

Read More

Thank you for your question and for your patience while I worked to have this antibody added to our catalog. The unconjugated form of ab3416 is now available as ab115044- http://www.abcam.com/GST-antibody-ab115044.html Please let me know if yo...

Read More

Sorry for the late response as the information you requested needed some double checking. I can confirm that the antibody preparation used for the FITC (ab3445) and HRP (ab3416) GST antibody is the same. I hope this confirmation will be helpful...

Read More

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up