SpecificityAssay by immunoelectrophoresis resulted in a single precipition arc against anti-Rabbit Serum, as well as purified and partially purified GST [Schistosoma japonicum]. Cross reactivity against GST from other sources may occur but has not been specifically determined.
Purification notesab106227 was prepared from monospecific antiserum by immunoaffinity
chromatography using GST coupled to agarose beads followed by solid
phase adsorption(s) to remove any unwanted reactivities and extensive
dialysis against the buffer.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2500.
1/2000 - 1/10000.
RelevanceGST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.