Overview

  • Product name
    GST tag ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8.5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 4%
  • Sample type
    Cell Lysate
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    1 ng/ml
  • Assay duration
    Multiple steps standard assay
  • Product overview

    Abcam’s Gluthathione S-Transferase (GST) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of GST tagged protein expression in cell extracts or after purification.

    The assay employs a GST specific antibody coated onto a well plate strips. Standards and samples are pipetted into the wells and GST present in the sample is bound to the wells by the immobilized antibody. The wells are washed and a primary anti- GST detector antibody is added. After washing away unbound detector antibody an HRP-conjugated secondary detector antibody (HRP Label) specific for the primary detector antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GST bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.

  • Notes

    Typical working ranges

    GST (pure protein) 1 – 200 ng/mL

    GST fusion protein 6 – 400ng/mL

     

    Gluthathione S-transferase (GST) is a 26 kDa protein typically used as a fusion partner of recombinant proteins to increase the level of protein expression in bacterial systems, enhance solubility, protect from proteolysis, improve folding and allow purification by affinity chromatography on gluthathione resin.

    This GST ELISA Kit is an enzyme immunoassay developed for the sensitive detection and quantitation of GST or GST fusion proteins in cells samples or purified tagged proteins. The quantity of GST can be determined in lysates or intermediate purification fractions by comparing their absorbance with that of a known recombinant GST standard curve. The kit has a detection sensitivity limit of 1 ng/mL of GST. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and GST fusion proteins.

  • Tested applications
    Suitable for: ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab126581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Example standard curve. GST standard protein was serially diluted from 400 ng/mL to 6 ng/mL. Background was subtracted from data points prior to log transformation and curve fitting (log-agonist vs. response – variable slope). Sensitivity was calculated mathematically to be as low as 1 ng/mL.
  • Dynamic Range of a recombinant protein tagged to GST. Recombinant full length protein (Human) p53 with N terminal GST tag (ab43615) was tested in a dilution series from 400 ng/mL to 12 ng/mL using the GST ELISA kit. Background was subtracted from data points prior to log transformation and curve fitting (log-agonist vs. response – variable slope).
  • All lanes : GST tag ELISA Kit (ab126581)

    Lane 1 : GST recombinant protein (ab89494)
    Lane 2 : p53 tagged to GST protein (ab43615)
  • All lanes : Anti-GST antibody (ab19256)

    Lane 1 : GST recombinant protein (ab89494)
    Lane 2 : p53 tagged to GST protein (ab43615)

Protocols

References

ab126581 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Gracias por contactarnos de nuevo.

Este kit ha sido diseñado para la medida cuantitativa del tag GST. Lamentablemente no se ha testado en muestras de suero.

Siento no poder ser de más ayuda, si hay algo mas en lo que p...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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