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Abcam’s Gluthathione S-Transferase (GST) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of GST tagged protein expression in cell extracts or after purification.
The assay employs a GST specific antibody coated onto a well plate strips. Standards and samples are pipetted into the wells and GST present in the sample is bound to the wells by the immobilized antibody. The wells are washed and a primary anti- GST detector antibody is added. After washing away unbound detector antibody an HRP-conjugated secondary detector antibody (HRP Label) specific for the primary detector antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GST bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
Typical working ranges
GST (pure protein) 1 – 200 ng/mL
GST fusion protein 6 – 400ng/mL
Gluthathione S-transferase (GST) is a 26 kDa protein typically used as a fusion partner of recombinant proteins to increase the level of protein expression in bacterial systems, enhance solubility, protect from proteolysis, improve folding and allow purification by affinity chromatography on gluthathione resin.
This GST ELISA Kit is an enzyme immunoassay developed for the sensitive detection and quantitation of GST or GST fusion proteins in cells samples or purified tagged proteins. The quantity of GST can be determined in lysates or intermediate purification fractions by comparing their absorbance with that of a known recombinant GST standard curve. The kit has a detection sensitivity limit of 1 ng/mL of GST. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and GST fusion proteins.
|Components||1 x 96 tests|
|10X Blocking Buffer||1 x 6ml|
|10X GST Detector Antibody||1 x 700µl|
|10X HRP Label||1 x 1ml|
|20X Buffer||1 x 20ml|
|Extraction Buffer||1 x 15ml|
|GST Microplate (12 x 8 antibody coated well strips)||1 unit|
|GST Standard||1 vial|
|HRP/TMB Development Solution||1 x 6ml|
Our Abpromise guarantee covers the use of ab126581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
ab126581 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"