Anti-GST3 / GST pi antibody (ab117885)

Overview

  • Product name
    Anti-GST3 / GST pi antibody
    See all GST3 / GST pi primary antibodies
  • Description
    Rabbit polyclonal to GST3 / GST pi
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human GST3/ GST pi.

  • Positive control
    • This antibody gave a positive signal in both Human Prostate and Kidney tissue lysates as well as the following whole cell lysates: PC3; HeLa; HEK293; Jurkat; K562. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal prostate. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7

Properties

Applications

Our Abpromise guarantee covers the use of ab117885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

Target

  • Function
    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
  • Sequence similarities
    Belongs to the GST superfamily. Pi family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deafness antibody
    • Deafness X-linked 7 antibody
    • DFN7 antibody
    • FAEES3 antibody
    • Fatty Acid Ethyl Ester Synthase III antibody
    • Glutathione S Transferase 3 antibody
    • Glutathione S Transferase Pi antibody
    • Glutathione S-transferase P antibody
    • Glutathione S-transferase pi 1 antibody
    • GST class-pi antibody
    • GST3 antibody
    • GSTP antibody
    • Gstp1 antibody
    • GSTP1-1 antibody
    • GSTP1_HUMAN antibody
    • PI antibody
    • X linked 7 antibody
    see all

Images



  • Predicted band size : 23 kDa

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: GST3 / GST pi knockout HAP1 whole cell lysate (20 µg) 
    Lane 3: Jurkat whole cell lysate (20 µg)
    Lane 4: Hek293 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab117885 observed at 23 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab117885 was shown to specifically react with GST3 / GST pi in wild-type HAP1 cells as signal was lost in GST3 / GST pi knockout cells. Wild-type and GST3 /GST pi knockout samples were subjected to SDS-PAGE. Ab117885 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-GST3 / GST pi antibody (ab117885) at 1 µg/ml

    Lane 1 : Human prostate tissue lysate (cirrhosis) - total protein (ab30307)
    Lane 2 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 6 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 7 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 23 kDa


    Exposure time : 30 seconds
  • IHC image of GST3 / GST pi staining in Human normal prostate formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117885, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab117885 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab117885 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab117885 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Zebrafish Cell (Whole mount Immunofluorescence)
Permeabilization
Yes - Methanol
Specification
Whole mount Immunofluorescence
Blocking step
2% BSA + 10% Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 20 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up