Anti-GST3 / GST pi antibody (ab150000)

Overview

  • Product name
    Anti-GST3 / GST pi antibody
    See all GST3 / GST pi primary antibodies
  • Description
    Rabbit polyclonal to GST3 / GST pi
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Goat, Horse, Cow, Dog, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human GST3/ GST pi.

  • Positive control
    • This antibody gave a positive signal in both Human Prostate and Kidney tissue lysates as well as the following whole cell lysates: PC3; HeLa; HEK293; Jurkat; K562. This antibody gave a positive result when used in the following formaldehyde fixed cell lines:HeLa

Properties

Applications

Our Abpromise guarantee covers the use of ab150000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

Target

  • Function
    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
  • Sequence similarities
    Belongs to the GST superfamily. Pi family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deafness antibody
    • Deafness X-linked 7 antibody
    • DFN7 antibody
    • FAEES3 antibody
    • Fatty Acid Ethyl Ester Synthase III antibody
    • Glutathione S Transferase 3 antibody
    • Glutathione S Transferase Pi antibody
    • Glutathione S-transferase P antibody
    • Glutathione S-transferase pi 1 antibody
    • GST class-pi antibody
    • GST3 antibody
    • GSTP antibody
    • Gstp1 antibody
    • GSTP1-1 antibody
    • GSTP1_HUMAN antibody
    • PI antibody
    • X linked 7 antibody
    see all

Images

  • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: GSTP1 (GST3 / GST pi) knockout  HAP1 whole cell lysate (20 µg)
    Lane 3: Jurkat whole cell lysate (20 µg)
    Lane 4: Hek293 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab150000 observed at 23 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab150000 was shown to specifically react with GST3 / GST pi in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when GST3 / GST pi knockout cells were examined. Wild-type and GST3 / GST pi knockout samples were subjected to SDS-PAGE. Ab150000 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-GST3 / GST pi antibody (ab150000) at 1 µg/ml

    Lane 1 : Human prostate tissue lysate (cirrhosis) - total protein (ab30307)
    Lane 2 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lane 6 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

    Lane 7 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 23 kDa
    Additional bands at: 140 kDa, 34 kDa, 82 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes
  • ab150000 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab150000 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab150000 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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This antibody has not been tested in dog, but it’s very likely it will cross react with this species, due to sequence homology.

If you are interested in testing ab150000 in dog samples, and get a f...

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