Human Predicted to work with:
Sheep, Cow, Pig, Cynomolgus monkey
Recombinant fragment: MAEKPKLHYS NIRGRMESIR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKEKA LIDMYIEGIA, corresponding to amino acids 1-100 of human GSTA2 (AAH02895) with a proprietary tag.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 26 kDa.
Use at an assay dependent dilution.
RelevanceCytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, which are located in a cluster on chromosome 6, are highly related and encode enzymes with glutathione peroxidase activity that function in the detoxification of lipid peroxidation products. Reactive electrophiles produced by oxidative metabolism have been linked to a number of degenerative diseases including Parkinson's disease, Alzheimer's disease, cataract formation, and atherosclerosis.
Anti-GSTA2 antibody (ab87967) at 5 µg/ml + human liver tissue lysate at 50 µg
Predicted band size : 26 kDa Observed band size : 26 kDa
Western blot - GSTA2 antibody (ab87967)
Anti-GSTA2 antibody (ab87967) at 5 µg/ml + HepG2 cell lysate at 50 µg
Predicted band size : 26 kDa Observed band size : 26 kDa Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
References for Anti-GSTA2 antibody (ab87967)
This product has been referenced in:
Lee LY et al. Activation of the Nrf2-ARE pathway in hepatocytes protects against steatosis in nutritionally induced non-alcoholic steatohepatitis in mice. Toxicol Sci142:361-74 (2014).
Read more (PubMed: 25294219) »