The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 26 kDa).
FunctionConjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This isozyme has a high catalytic efficiency with 4-hydroxyalkenals such as 4-hydroxynonenal (4-HNE).
Tissue specificityExpressed at a high level in brain, placenta, and skeletal muscle and much lower in lung and liver.
Sequence similaritiesBelongs to the GST superfamily. Alpha family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Malhotra D et al. Global mapping of binding sites for Nrf2 identifies novel targets in cell survival response through ChIP-Seq profiling and network analysis. Nucleic Acids Res38:5718-34 (2010).
Read more (PubMed: 20460467) »