Overview

  • Product name
    Anti-GSTM1 + GSTM2 antibody
  • Description
    Goat polyclonal to GSTM1 + GSTM2
  • Specificity
    This antibody is expected to recognise both reported variants of human GSTM1 protein (NP_000552; NP_666533) and GSTM2 protein (NP_000848).
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus monkey
  • Immunogen

    Synthetic peptide:

    CVFSKMAVWGNK

    from the C Terminus of the protein (Human).

  • Positive control
    • Human liver and lung lysates for Western blot.

Properties

Applications

Our Abpromise guarantee covers the use of ab27489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

Target

  • Relevance
    Glutathione S transferases are encoded by two distinct supergene families and a total of eight distinct classes of the soluble cytoplasmic mammalian glutathione S transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. The mu class of these enzymes, to which GSTM1 and 2 belong, functions in the detoxification of electrophilic compounds by conjugation with glutathione. The genes encoding the mu class of enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs and null mutations of this gene have been linked with an increase in a number of cancers.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • Glutathione S alkyltransferase M1 antibody
    • Glutathione S alkyltransferase M2 antibody
    • Glutathione S transferase M1 antibody
    • Glutathione S transferase Mu 1 antibody
    • Glutathione S transferase Mu 2 antibody
    • GST class mu 1 antibody
    • GSTM antibody
    • GTH4 antibody
    • GTM1 antibody
    • S(hydroxyalkyl) glutathione lyase antibody
    see all

Images

  • Anti-GSTM1 + GSTM2 antibody (ab27489) at 0.3 µg/ml + human lung lysate (35µg protein in RIPA buffer).

    Predicted band size : 26 kDa
    Observed band size : 26 kDa

References

This product has been referenced in:
  • Shan Y  et al. Frataxin deficiency leads to defects in expression of antioxidants and Nrf2 expression in dorsal root ganglia of the Friedreich's ataxia YG8R mouse model. Antioxid Redox Signal 19:1481-93 (2013). Read more (PubMed: 23350650) »
  • Yang Y  et al. Glutathione S-Transferase-{mu}1 Regulates Vascular Smooth Muscle Cell Proliferation, Migration, and Oxidative Stress. Hypertension : (2009). Read more (PubMed: 19822795) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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