• Product name
  • Description
    Rabbit polyclonal to GSTM3
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cow, Pig
  • Immunogen

    A synthetic peptide corresponding to a region of human GSTM3

  • Positive control
    • HepG2 cell lysate This antibody gave a positive result when used in the following methanol fixed cell line: HeLa.



Our Abpromise guarantee covers the use of ab74749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.


  • Function
    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. May govern uptake and detoxification of both endogenous compounds and xenobiotics at the testis and brain blood barriers.
  • Tissue specificity
    Testis and brain.
  • Sequence similarities
    Belongs to the GST superfamily. Mu family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.
  • Post-translational
    The N-terminus is blocked.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • brain GST antibody
    • brain type mu glutathione S transferase antibody
    • glutathione S alkyltransferase M3 antibody
    • glutathione S aryltransferase M3 antibody
    • glutathione S transferase M3 (brain) antibody
    • glutathione S transferase M3 antibody
    • Glutathione S transferase Mu 3 antibody
    • glutathione S transferase, Mu 3 antibody
    • Glutathione S-transferase Mu 3 antibody
    • GST class mu 3 antibody
    • GST class-mu 3 antibody
    • GST5 antibody
    • GSTB antibody
    • Gstm3 antibody
    • GSTM3-3 antibody
    • GSTM3_HUMAN antibody
    • GTM3 antibody
    • hGSTM3-3 antibody
    • MGC3310 antibody
    • MGC3704 antibody
    • S (hydroxyalkyl)glutathione lyase M3 antibody
    see all


  • ICC/IF image of ab74749 stained HeLa cells.  The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum/ 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab74749 at 5 µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-GSTM3 antibody (ab74749) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 27 kDa
    Observed band size : 27 kDa


This product has been referenced in:
  • Kocevar N  et al. Proteomic analysis of gastric cancer and immunoblot validation of potential biomarkers. World J Gastroenterol 18:1216-28 (2012). WB ; Human . Read more (PubMed: 22468085) »
  • Scantland S  et al. Method to isolate polyribosomal mRNA from scarce samples such as mammalian oocytes and early embryos. BMC Dev Biol 11:8 (2011). WB ; Cow . Read more (PubMed: 21324132) »

See all 2 Publications for this product

Customer reviews and Q&As

You have been using the product at a very low concentration (1/10,000). We recommend on our datasheet to use at a starting concentration of 1/1000, in this particular case you may find that you may need an even higher concentration and we recommend to ...

Read More

The short peptide sequence that was used to raise this antibody is 14aa in length, 100% homologous to this protein (http://www.uniprot.org/uniprot/P21266) and contained within this larger 50aa sequence: EEKIRVDIIENQVMDFRTQLIRLCYSSDHEKLKPQYLEELPGQLKQFSM...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Tissue lysate - whole (stomach)
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12 %)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

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Verified customer

Submitted Jul 11 2011


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