The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
Use at an assay dependent dilution. Detection limit for this antibody is 0.3ng/ml as a capture antibody when tested against the immunogen.
FunctionTFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.
Sequence similaritiesBelongs to the TFIIA subunit 2 family.