Overview

  • Product nameAnti-GTF2F2 antibody
    See all GTF2F2 primary antibodies
  • Description
    Rabbit polyclonal to GTF2F2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 200-249 (KDLVDITKQP VVYLKEILKE IGVQNVKGIH KNTWELKPEY RHYQGEEKSD) of Human GTF2F2 (NP_004119).

  • Positive control
    • Human fetal liver lysate; Human kidney tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab105133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionTFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity.
  • Sequence similaritiesBelongs to the TFIIF beta subunit family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent helicase GTF2F2 antibody
    • ATP-dependent helicase GTF2F2 antibody
    • BTF4 antibody
    • General transcription factor IIF 30 kDa subunit antibody
    • General transcription factor IIF polypeptide 2 antibody
    • General transcription factor IIF subunit 2 antibody
    • General transcription factor IIF, polypeptide 2, 30kDa antibody
    • Gtf2f2 antibody
    • RAP30 antibody
    • T2FB_HUMAN antibody
    • TF2F2 antibody
    • TFIIF antibody
    • TFIIF beta antibody
    • TFIIF-beta antibody
    • Transcription initiation factor IIF subunit beta antibody
    • Transcription initiation factor RAP30 antibody
    see all

Anti-GTF2F2 antibody images

  • Anti-GTF2F2 antibody (ab105133) at 0.5 µg/ml + Human fetal liver lysate at 10 µg

    Predicted band size : 28 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling GTF2F2 with ab105133 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

References for Anti-GTF2F2 antibody (ab105133)

ab105133 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"