Overview

  • Product name
  • Description
    Mouse polyclonal to GTF2H2
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Full length protein, corresponding to amino acids 1-395 of Human GTF2H2 (NP_001506.1).

  • Positive control
    • HepG2 cells; GTF2H2 transfected 293T cell lysate; Human pancreas tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab169288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. The N-terminus interacts with and regulates XPD whereas an intact C-terminus is required for a successful escape of RNAP II form the promoter.
  • Tissue specificity
    Widely expressed, with higher expression in skeletal muscle.
  • Sequence similarities
    Belongs to the GTF2H2 family.
    Contains 1 VWFA domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic transcription factor 2 44 kDa subunit antibody
    • BTF2 antibody
    • BTF2 p44 antibody
    • BTF2-p44 antibody
    • BTF2P44 antibody
    • General transcription factor IIH antibody
    • General transcription factor IIH polypeptide 2 antibody
    • General transcription factor IIH subunit 2 antibody
    • General transcription factor IIH, polypeptide 2, 44kDa antibody
    • Gtf2h2 antibody
    • MGC102806 antibody
    • p44 antibody
    • T BTF2P44 antibody
    • T-BTF2P44 antibody
    • TF2H2_HUMAN antibody
    • TFIIH antibody
    • TFIIH basal transcription factor complex p44 subunit antibody
    see all

Images

  • Immunofluorescent analysis of HepG2 cells labeling GTF2H2 with ab169288 at 10µg/ml.
  • All lanes : Anti-GTF2H2 antibody (ab169288) at 1 µg/ml

    Lane 1 : GTF2H2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa

  • Anti-GTF2H2 antibody (ab169288) at 1 µg/ml + Human pancreas tissue lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa

References

This product has been referenced in:
  • Terasaki K  et al. Mechanistic Insight into the Host Transcription Inhibition Function of Rift Valley Fever Virus NSs and Its Importance in Virulence. PLoS Negl Trop Dis 10:e0005047 (2016). Read more (PubMed: 27711108) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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