Anti-GTF2H2C antibody - C-terminal (ab170429)

Overview

  • Product name
    Anti-GTF2H2C antibody - C-terminal
  • Description
    Rabbit polyclonal to GTF2H2C - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human GTF2H2C aa 321-351 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q6P1K8

  • Positive control
    • HeLa, 293, NCI-H460, NCI-H292, and A2058 cell lysates; Human prostate carcinoma tissue; NCI-H460 cells.
  • General notes

    This product will cross-react with GTF2H2 (SwissProt ID = Q13888).

Properties

Applications

Our Abpromise guarantee covers the use of ab170429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 44 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/10 - 1/50.

Target

  • Function
    Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II.
  • Sequence similarities
    Belongs to the GTF2H2 family.
    Contains 1 VWFA domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • General transcription factor IIH polypeptide 2-like protein antibody
    • General transcription factor IIH polypeptide 2C antibody
    • General transcription factor IIH subunit 2-like protein antibody
    • GTF2H2D antibody
    • T2H2L_HUMAN antibody
    see all

Images

  • All lanes : Anti-GTF2H2C antibody - C-terminal (ab170429) at 1/100 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : NCI-H460 cell lysate
    Lane 4 : NCI-H292 cell lysate
    Lane 5 : A2058 cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 44 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue labeling GTF2H2 with ab170429 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Confocal immunofluorescent analysis of NCI-H460 cells labeling GTF2H2 with ab170429 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

References

ab170429 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab170429.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up