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Synthetic peptide within Human GTF2H2C aa 321-351 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q6P1K8
This product will cross-react with GTF2H2 (SwissProt ID = Q13888).
Our Abpromise guarantee covers the use of ab170429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Predicted molecular weight: 44 kDa.|
|IHC-P||1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/10 - 1/50.|
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue labeling GTF2H2 with ab170429 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of NCI-H460 cells labeling GTF2H2 with ab170429 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
ab170429 has not yet been referenced specifically in any publications.