Overview

  • Product name
  • Description
    Rabbit polyclonal to GTF2IRD1
  • Specificity
    This antibody detects endogenous levels of total GTF2IRD1 protein.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthetic peptide derived from N terminus of human GTF2IRD1.

  • Positive control
    • Extracts from 293 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab64805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
ELISA 1/20000.

Target

  • Function
    May be a transcription regulator involved in cell-cycle progression and skeletal muscle differentiation. May repress GTF2I transcriptional functions, by preventing its nuclear residency, or by inhibiting its transcriptional activation. May contribute to slow-twitch fiber type specificity during myogenesis and in regenerating muscles. Binds troponin I slow-muscle fiber enhancer (USE B1). Binds specifically and with high affinity to the EFG sequences derived from the early enhancer of HOXC8.
  • Tissue specificity
    Highly expressed in adult skeletal muscle, heart, fibroblast, bone and fetal tissues. Expressed at lower levels in all other tissues tested.
  • Involvement in disease
    Note=GTF2IRD1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of GTF2IRD1 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • Sequence similarities
    Belongs to the TFII-I family.
    Contains 5 GTF2I-like repeats.
  • Developmental stage
    Highly expressed in developing and regenerating muscles, at the time of myofiber diversification.
  • Domain
    The N-terminal half may have an activating activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CREAM1 antibody
    • General transcription factor II I repeat domain containing protein 1 antibody
    • General transcription factor II-I repeat domain-containing protein 1 antibody
    • General transcription factor III antibody
    • GT2D1_HUMAN antibody
    • GTF2I repeat domain containing protein 1 antibody
    • GTF2I repeat domain-containing protein 1 antibody
    • GTF2IRD1 antibody
    • GTF3 antibody
    • Muscle TFII I repeat domain-containing protein 1 antibody
    • Muscle TFII-I repeat domain-containing protein 1 antibody
    • MUSTRD1 antibody
    • MusTRD1/BEN antibody
    • RBAP2 antibody
    • Slow muscle fiber enhancer binding protein antibody
    • Slow-muscle-fiber enhancer-binding protein antibody
    • USE B1 binding protein antibody
    • USE B1-binding protein antibody
    • WBSCR11 antibody
    • WBSCR12 antibody
    • Williams Beuren syndrome chromosome region 11 protein antibody
    • Williams-Beuren syndrome chromosomal region 11 protein antibody
    • Williams-Beuren syndrome chromosomal region 12 protein antibody
    see all

Images

  • All lanes : Anti-GTF2IRD1 antibody (ab64805) at 1/500 dilution

    Lane 1 : Extracts from 293 cells, no peptide
    Lane 2 : Extracts from 293 cells, plus peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 106 kDa
    Observed band size : 106 kDa

References

This product has been referenced in:
  • Masuda T  et al. The transcription factor GTF2IRD1 regulates the topology and function of photoreceptors by modulating photoreceptor gene expression across the retina. J Neurosci 34:15356-68 (2014). WB, IP, IHC ; Mouse . Read more (PubMed: 25392503) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (spleen/ lymphnotes)
Loading amount
1e+006 cells
Specification
spleen/ lymphnotes
Treatment
antiCD3/CD28 for 7h
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
non vertebrate (Licor) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
Username

Ms. Jargosch

Verified customer

Submitted Feb 16 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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