Overview

  • Product nameAnti-GTF3A antibody
    See all GTF3A primary antibodies
  • Description
    Rabbit polyclonal to GTF3A
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human GTF3A.

    (Peptide available as ab90596.)

  • Positive control
    • This antibody gave a positive signal in human testis and liver tissue lysates as well as the following whole cell lysates: HeLa; Jurkat; HepG2.

Properties

Applications

Our Abpromise guarantee covers the use of ab76894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 41 kDa).

Target

  • FunctionInteracts with the internal control region (ICR) of approximately 50 bases within the 5S RNA genes, is required for correct transcription of these genes by RNA polymerase III. Also binds the transcribed 5S RNA's. May initiate transcription of the 5S ribosomal RNA gene and maintain the stability of transcription of other genes.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesContains 9 C2H2-type zinc fingers.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AP 2 antibody
    • AP2 antibody
    • Factor A antibody
    • General transcription factor 3A antibody
    • General transcription factor IIIA antibody
    • GTF 3A antibody
    • GTF3A antibody
    • TF3A_HUMAN antibody
    • TFIIIA antibody
    • Transcription factor 3A antibody
    • Transcription factor IIIA antibody
    see all

Anti-GTF3A antibody images

  • All lanes : Anti-GTF3A antibody (ab76894) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : Human liver tissue lysate - total protein (ab29889)
    Lane 5 : Human testis tissue lysate - total protein (ab30257)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa,45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

References for Anti-GTF3A antibody (ab76894)

ab76894 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing
Sample Sheep Cell lysate - whole cell (ovine B cell line)
Specification ovine B cell line
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Feb 20 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"