Anti-GTF3C2 antibody - ChIP Grade (ab89113)

Overview

  • Product name
    Anti-GTF3C2 antibody - ChIP Grade
    See all GTF3C2 primary antibodies
  • Description
    Mouse polyclonal to GTF3C2 - ChIP Grade
  • Tested applications
    Suitable for: ChIP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length GTF3C2 protein (NP_001030598.1).

  • Positive control
    • HeLa cell lysate, GTF3C2 transfected 293T cell line lysate and HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab89113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP 1/100.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 101 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Required for RNA polymerase III-mediated transcription. Component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. May play a direct role in stabilizing interactions of TFIIIC2 with TFIIIC1.
  • Sequence similarities
    Contains 4 WD repeats.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • General transcription factor 3C polypeptide 2 antibody
    • General transcription factor IIIC polypeptide 2 beta 110kDa antibody
    • Gtf3c2 antibody
    • KIAA0011 antibody
    • TF3C beta antibody
    • TF3C-beta antibody
    • TF3C2_HUMAN antibody
    • TFIIIC 110 kDa subunit antibody
    • TFIIIC BETA antibody
    • TFIIIC110 antibody
    • Transcription factor IIIC 110 kDa subunit antibody
    • Transcription factor IIIC subunit beta antibody
    see all

Images

  • ChIP analysis using ab89113 binding GTF3C2 in iPS derived from Human neuronal. Cells were cross-linked for 10 minutes with  formaldehyde. Samples were incubated with primary antibody(1/100) for 16 hours at 4°C. Protein binding was detected using real-time PCR.
    Positive control: 7SL RNA gene

    Negative Control: GAPDH coding region.

     

    See Abreview

  • Anti-GTF3C2 antibody - ChIP Grade (ab89113) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 101 kDa
    Observed band size : 101 kDa
  • All lanes : Anti-GTF3C2 antibody - ChIP Grade (ab89113) at 1 µg/ml

    Lane 1 : GTF3C2 transfected 293T cell line lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 101 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
  • ab89113, at 10 µg/ml staining GTF3C2 in HeLa cells by Immunofluorescence.

References

ab89113 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (Gradientgel 4-12)
Sample
Mouse Cell lysate - whole cell (Hela,HEK293, fibroblasts, ES cells)
Specification
Hela,HEK293, fibroblasts, ES cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Oct 14 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Human Cell lysate - whole cell (iPS derived neuronal cells)
Specification
iPS derived neuronal cells
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step
Real-time PCR
Positive control
7SL RNA gene
Negative control
GAPDH coding region
Username

Abcam user community

Verified customer

Submitted Apr 11 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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