The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 101 kDa.
Use a concentration of 10 µg/ml.
FunctionRequired for RNA polymerase III-mediated transcription. Component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. May play a direct role in stabilizing interactions of TFIIIC2 with TFIIIC1.
ChIP - Anti-GTF3C2 antibody (ab89113)This image is courtesy of an anonymous Abreview
ChIP analysis using ab89113 binding GTF3C2 in iPS derived from Human neuronal. Cells were cross-linked for 10 minutes with formaldehyde. Samples were incubated with primary antibody(1/100) for 16 hours at 4°C. Protein binding was detected using real-time PCR. Positive control: 7SL RNA gene