Anti-TFIIIC110 antibody (ab89113)
Key features and details
- Mouse polyclonal to TFIIIC110
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TFIIIC110 antibody -
Description
Mouse polyclonal to TFIIIC110 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human TFIIIC110. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 101 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 101 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Required for RNA polymerase III-mediated transcription. Component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. May play a direct role in stabilizing interactions of TFIIIC2 with TFIIIC1. -
Sequence similarities
Contains 4 WD repeats. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2976 Human
- Omim: 604883 Human
- SwissProt: Q8WUA4 Human
- Unigene: 75782 Human
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Alternative names
- General transcription factor 3C polypeptide 2 antibody
- General transcription factor IIIC polypeptide 2 beta 110kDa antibody
- Gtf3c2 antibody
see all
Images
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Anti-TFIIIC110 antibody (ab89113) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 101 kDa
Observed band size: 101 kDa -
All lanes : Anti-TFIIIC110 antibody (ab89113) at 1 µg/ml
Lane 1 : GTF3C2 transfected 293T cell line lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 101 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted? -
ab89113, at 10 µg/ml staining TFIIIC110 in HeLa cells by Immunofluorescence.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab89113 has been referenced in 3 publications.
- Ferrari R et al. TFIIIC Binding to Alu Elements Controls Gene Expression via Chromatin Looping and Histone Acetylation. Mol Cell 77:475-487.e11 (2020). PubMed: 31759822
- Khattar E et al. Telomerase reverse transcriptase promotes cancer cell proliferation by augmenting tRNA expression. J Clin Invest 126:4045-4060 (2016). PubMed: 27643433
- Wang J et al. Cytoskeletal Filamin A Differentially Modulates RNA Polymerase III Gene Transcription in Transformed Cell Lines. J Biol Chem 291:25239-25246 (2016). PubMed: 27738102