Overview

  • Product name
    Anti-gtf3c3 antibody
  • Description
    Rabbit polyclonal to gtf3c3
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to a region between residue 475 and 525 of human gtf3c3 (NP_036218.1)

  • Positive control
    • HeLa whole cell lysate. Mouse NIH3T3 whole Cell Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab74230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 101 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Involved in RNA polymerase III-mediated transcription. Integral, tightly associated component of the DNA-binding TFIIIC2 subcomplex that directly binds tRNA and virus-associated RNA promoters.
  • Sequence similarities
    Contains 11 TPR repeats.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • General transcription factor 3C polypeptide 3 antibody
    • general transcription factor IIIC, polypeptide 3 (102kD) antibody
    • general transcription factor IIIC, polypeptide 3, 102kD antibody
    • GTF3C3 antibody
    • TF3C-gamma antibody
    • TF3C3_HUMAN antibody
    • TFIIIC 102 kDa subunit antibody
    • TFIIIC gamma antibody
    • TFIIIC102 antibody
    • TFIIIC2 102 antibody
    • TFIIICgamma antibody
    • Transcription factor IIIC 102 kDa subunit antibody
    • transcription factor IIIC gamma subunit antibody
    • Transcription factor IIIC subunit gamma antibody
    • transcription factor IIIC, 102 kD antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling gtf3c3 with ab74230 at 1/500 (0.4µg/ml). Detection: DAB.
  • All lanes : Anti-gtf3c3 antibody (ab74230) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cell at 50 µg
    Lane 5 : mouse NIH3T3 cell at 50 µg


    Predicted band size : 101 kDa
    Observed band size : 101 kDa


    Exposure time : 3 minutes
  • Lane 1: HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) with ab74230 used for IP at 3 µg/mg lysate.
    Lane 2: control IgG.
    Subsequent Western blot detection peformed using ab74230 at 1 µg/ml.
    Detection: Chemiluminescence with an exposure time of 3 minutes.

References

ab74230 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab74230.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up