• Product nameAnti-gtf3c3 antibody
  • Description
    Rabbit polyclonal to gtf3c3
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to a region between residue 475 and 525 of human gtf3c3 (NP_036218.1)

  • Positive control
    • HeLa whole cell lysate. Mouse NIH3T3 whole Cell Lysate.



Our Abpromise guarantee covers the use of ab74230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 101 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • FunctionInvolved in RNA polymerase III-mediated transcription. Integral, tightly associated component of the DNA-binding TFIIIC2 subcomplex that directly binds tRNA and virus-associated RNA promoters.
  • Sequence similaritiesContains 11 TPR repeats.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • General transcription factor 3C polypeptide 3 antibody
    • general transcription factor IIIC, polypeptide 3 (102kD) antibody
    • general transcription factor IIIC, polypeptide 3, 102kD antibody
    • GTF3C3 antibody
    • TF3C-gamma antibody
    • TF3C3_HUMAN antibody
    • TFIIIC 102 kDa subunit antibody
    • TFIIIC gamma antibody
    • TFIIIC102 antibody
    • TFIIIC2 102 antibody
    • TFIIICgamma antibody
    • Transcription factor IIIC 102 kDa subunit antibody
    • transcription factor IIIC gamma subunit antibody
    • Transcription factor IIIC subunit gamma antibody
    • transcription factor IIIC, 102 kD antibody
    see all

Anti-gtf3c3 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling gtf3c3 with ab74230 at 1/500 (0.4µg/ml). Detection: DAB.
  • All lanes : Anti-gtf3c3 antibody (ab74230) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cell at 50 µg
    Lane 5 : mouse NIH3T3 cell at 50 µg

    Predicted band size : 101 kDa
    Observed band size : 101 kDa

    Exposure time : 3 minutes
  • Lane 1: HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) with ab74230 used for IP at 3 µg/mg lysate.
    Lane 2: control IgG.
    Subsequent Western blot detection peformed using ab74230 at 1 µg/ml.
    Detection: Chemiluminescence with an exposure time of 3 minutes.

References for Anti-gtf3c3 antibody (ab74230)

ab74230 has not yet been referenced specifically in any publications.

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