Overview

  • Product nameAnti-GTF3C4 antibody
    See all GTF3C4 primary antibodies
  • Description
    Rabbit polyclonal to GTF3C4
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1 and 50 of human GTF3C4 (NP_036336.1)

  • Positive control
    • HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab74229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 92 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionEssential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box.
  • Sequence similaritiesBelongs to the TFIIIC subunit 4 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.3.1.48 antibody
    • FLJ21002 antibody
    • General transcription factor 3C 4 antibody
    • General transcription factor 3C polypeptide 4 antibody
    • General transcription factor IIIC, polypeptide 4, 90kDa antibody
    • GTF3C4 antibody
    • KAT12 antibody
    • MGC138450 antibody
    • OTTHUMP00000022434 antibody
    • TF3C delta antibody
    • TF3C-delta antibody
    • TF3C4_HUMAN antibody
    • TFIII90 antibody
    • TFIIIC 90 kDa subunit antibody
    • TFiiiC2 90 antibody
    • TFIIIC290 antibody
    • TFIIIC90 antibody
    • TFIIICdelta antibody
    • Transcription factor IIIC 90 kDa subunit antibody
    • Transcription factor IIIC subunit delta antibody
    see all

Anti-GTF3C4 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue labelling GTF3C4 with ab74229 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-GTF3C4 antibody (ab74229) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 92 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 185 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 seconds
  • ab74229, at 1 µg/ml (used for IP at 3 µg/mg lysate)
    Lane 1: HeLa whole cell lysate at 1 mg for IP (20% of IP loaded).
    Lane 2: control IgG.
    Detection: Chemiluminescence with an exposure time of 3 seconds.

References for Anti-GTF3C4 antibody (ab74229)

ab74229 has not yet been referenced specifically in any publications.

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