1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box.
ab74229, at 1 µg/ml (used for IP at 3 µg/mg lysate)
Lane 1: HeLa whole cell lysate at 1 mg for IP (20% of IP loaded).
Lane 2: control IgG.
Detection: Chemiluminescence with an exposure time of 3 seconds.