1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box.
Flow cytometric analysis of HeLa cells labeling GTF3C4 with ab170386 at 1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.
Western blot - Anti-GTF3C4 antibody - N-terminal (ab170386)
Anti-GTF3C4 antibody - N-terminal (ab170386) at 1/100 dilution + NIH-3T3 cell lysate at 35 µg