Overview

  • Product nameAnti-GTF3C5 antibody
    See all GTF3C5 primary antibodies
  • Description
    Rabbit polyclonal to GTF3C5
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • Immunogen

    Synthetic peptide from a region between residue 469 and 519 of human GTF3C5 (using the numbering given in entry NP_036219.1)

  • Positive control
    • Lysate from HeLa, 293T or NIH3T3 cells

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab74305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 60 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-GTF3C5 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling GTF3C5 with ab74305 at 1/500 (0.4µg/ml). Detection: DAB.
  • All lanes : Anti-GTF3C5 antibody (ab74305) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
  • 20% of immunoprecipitate obtained from 1mg HeLa whole cell lysate using 3µg ab74305 or a control IgG was loaded in lane 1 and 2 respectively. GTF3C5 was then detected by WB, using ab74305 at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

References for Anti-GTF3C5 antibody (ab74305)

ab74305 has not yet been referenced specifically in any publications.

Product Wall

Application ChIP
Detection step Real-time PCR
Sample Mouse Cell lysate - whole cell (NIH 3T3)
Specification NIH 3T3
Negative control RNA Pol II genes
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Positive control RNA Pol III genes
Username

Ms. Jennifer Blancas

Verified customer

Submitted Jul 14 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"