Overview

  • Product nameAnti-GTL3 antibody
  • Description
    Rabbit polyclonal to GTL3
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide derived from a region between residues 144 - 193 of human GTL3.

  • Positive control
    • Placenta lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab33872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/62500.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-GTL3 antibody images

  • Anti-GTL3 antibody (ab33872) at 1 µg/ml (in 5% skim milk/PBS buffer) + Placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-rabbit IgG at 1/50000 dilution

    Predicted band size : 23 kDa
    Observed band size : 23 kDa

References for Anti-GTL3 antibody (ab33872)

This product has been referenced in:
  • Mendes Maia T  et al. Bug22 influences cilium morphology and the post-translational modification of ciliary microtubules. Biol Open 3:138-51 (2014). WB ; Drosophila melanogaster . Read more (PubMed: 24414207) »

See 1 Publication for this product

Product Wall

Abreviews
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH-3T3 cells)
Gel Running Conditions Reduced Denaturing (12% Tris-Glycine)
Loading amount 50 µg
Specification NIH-3T3 cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

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Verified customer

Submitted Nov 18 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"