Anti-GTP binding protein era homolog antibody (ab50874)

Overview

  • Product nameAnti-GTP binding protein era homolog antibody
    See all GTP binding protein era homolog primary antibodies
  • Description
    Rabbit polyclonal to GTP binding protein era homolog
  • SpecificityThis antibody reacts with GTP binding protein era homolog
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    A region within synthetic peptide: KTAVWEEGPG GELVIQQKLL VPKESYVKLL IGPKGHVISQ IAQEAGHDLM, corresponding to amino acids 371-420 of Human GTP binding protein era homolog

  • Positive control
    • HepG2 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab50874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 48 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:62500.

Target

  • FunctionProbable GTPase that plays a role in the mitochondrial ribosomal small subunit assembly. Specifically binds the 12S mitochondrial rRNA (12S mt-rRNA) to a 33 nucleotide section delineating the 3' terminal stem-loop region. May act as a chaperone that protects the 12S mt-rRNA on the 28S mitoribosomal subunit during ribosomal small subunit assembly.
  • Sequence similaritiesBelongs to the Era/MnmE GTP-binding protein family. Era subfamily.
    Contains 1 G (guanine nucleotide-binding) domain.
    Contains 1 KH type-2 domain.
  • Cellular localizationMitochondrion matrix. Mitochondrion inner membrane. Localizes on the matrix side on the mitochondrial inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CEGA antibody
    • Conserved ERA like GTPase antibody
    • Conserved ERA-like GTPase antibody
    • ERA antibody
    • Era (E. coli G protein homolog) like 1 antibody
    • Era G protein like 1 (E. coli) antibody
    • Era G protein like 1 antibody
    • ERA W antibody
    • ERA-like protein 1 antibody
    • ERA-W antibody
    • Eral1 antibody
    • ERAL1_HUMAN antibody
    • ERAL1A antibody
    • ERAW antibody
    • GTP binding protein era homolog antibody
    • GTPase Era antibody
    • GTPase Era, mitochondrial antibody
    • GTPase human homolog of E. coli essential cell cycle protein Era antibody
    • H-ERA antibody
    • HERA A antibody
    • hERA antibody
    • HERA B antibody
    • HERAA antibody
    • HERAB antibody
    • mitochondrial antibody
    see all

Anti-GTP binding protein era homolog antibody images

  • Anti-GTP binding protein era homolog antibody (ab50874) at 0.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

References for Anti-GTP binding protein era homolog antibody (ab50874)

ab50874 has not yet been referenced specifically in any publications.

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