The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 48 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1:62500.
FunctionProbable GTPase that plays a role in the mitochondrial ribosomal small subunit assembly. Specifically binds the 12S mitochondrial rRNA (12S mt-rRNA) to a 33 nucleotide section delineating the 3' terminal stem-loop region. May act as a chaperone that protects the 12S mt-rRNA on the 28S mitoribosomal subunit during ribosomal small subunit assembly.
Sequence similaritiesBelongs to the Era/MnmE GTP-binding protein family. Era subfamily. Contains 1 G (guanine nucleotide-binding) domain. Contains 1 KH type-2 domain.
Cellular localizationMitochondrion matrix. Mitochondrion inner membrane. Localizes on the matrix side on the mitochondrial inner membrane.