GTPase HRAS antibody [H-Ras-03] - Activation site (ab64705)

Product Name 

GTPase HRAS antibody [H-Ras-03] - Activation site

Product type 

Primary antibodies

Description 

Mouse monoclonal [H-Ras-03] to GTPase HRAS - Activation site

Immunogen 

Synthetic peptide: DIHQYREQIKRVKDSDDC, corresponding to internal sequence amino acids 92-109 of Human GTPase HRAS
BLAST 'DIHQYREQIKRVKDSDDC' with or

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Rat, Chmp, Hrs, Op, Zfsh
(Due to sequence homology)

Tested applications 
(see key)

ELISA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

RAJI human Burkitt lymphoma cell line
HeLa human cervix carcinoma cell line
K562 human Caucasian chronic myeloid leukemia cell line

Cellular localization 

Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Note: Shuttles between the plasma membrane and the Golgi apparatus.

Research areas 

Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Second Messenger >> Nucleotide Messenger >> GTP
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins

Relevance 

RAS proteins are signal-transducing, guanine nucleotide-binding proteins that appear to function as a branchpoint in signal transduction. RAS coordinates the activity of multiple signalling pathways, regulating diverse cellular functions including cell growth, differentiation and apoptosis. The human RAS gene family consists of three identified members which encode proteins of 21 kDa. Human cH RAS and cK RAS are the cellular homologs of vH- and vK RAS originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. The third family member is designated cN RAS. Normal cellular ras genes are referred to as proto-oncogenes and have the potential for activation to oncogenes by mutations occurring in codons 12, 13 and 61. Such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

H-Ras-03

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

ab64705 was purified from ascites by protein-A affinity chromatography. Purity is >95% by SDS-PAGE.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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