Overview

  • Product name
  • Description
    Rabbit polyclonal to GTPBP4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 339-613 of Human GTPBP4 (UniProt: Q9BZE4).

  • Positive control
    • 293T, A431, HeLa, HepG2, NIH 3T3, JC and BCL1 whole cell lysates; A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab153977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 74 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Involved in the biogenesis of the 60S ribosomal subunit.
  • Sequence similarities
    Belongs to the GTP1/OBG family. NOG subfamily.
  • Cellular localization
    Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chronic renal failure gene protein antibody
    • CRFG antibody
    • G protein binding protein CRFG antibody
    • GTP binding protein 4 antibody
    • GTP-binding protein NGB antibody
    • Gtpbp4 antibody
    • NGB antibody
    • NOG1 antibody
    • NOG1_HUMAN antibody
    • Nucleolar GTP binding protein 1 antibody
    • Nucleolar GTP-binding protein 1 antibody
    see all

Images

  • All lanes : Anti-GTPBP4 antibody (ab153977) at 1/5000 dilution

    Lane 1 : NIH 3T3 whole cell lysate
    Lane 2 : JC whole cell lysate
    Lane 3 : BCL1 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 74 kDa



    7.5% SDS PAGE
  • All lanes : Anti-GTPBP4 antibody (ab153977) at 1/10000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 74 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of A431 cells (fixed in iced-cold MeOH for 5 min) labeling GTPBP4 with ab153977 at 1/500 dilution (green). Right image shows cells co-stained with Hoechst 33342 (blue).

References

ab153977 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab153977.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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