Overview

  • Product nameAnti-GTPBP4 antibody [EPR3500]
    See all GTPBP4 primary antibodies
  • Description
    Rabbit monoclonal [EPR3500] to GTPBP4
  • Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human GTPBP4.

  • Positive control
    • Human fetal kidney, fetal brain, Human placenta and Human testis tissue lysates; Human kidney tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab92342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
IHC-P
Flow Cyt
  • Application notesFlow Cyt: 1/50.
    IHC-P: 1/250 - 1/500. Antigen retrieval is recommended.
    IP: 1/50.
    WB: 1/2000 - 1/5000. Detects a band of approximately 80 kDa (predicted molecular weight: 74 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in the biogenesis of the 60S ribosomal subunit.
    • Sequence similaritiesBelongs to the GTP1/OBG family. NOG subfamily.
    • Cellular localizationNucleus > nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Chronic renal failure gene protein antibody
      • CRFG antibody
      • G protein binding protein CRFG antibody
      • GTP binding protein 4 antibody
      • GTP-binding protein NGB antibody
      • Gtpbp4 antibody
      • NGB antibody
      • NOG1 antibody
      • NOG1_HUMAN antibody
      • Nucleolar GTP binding protein 1 antibody
      • Nucleolar GTP-binding protein 1 antibody
      see all

    Anti-GTPBP4 antibody [EPR3500] images

    • All lanes : Anti-GTPBP4 antibody [EPR3500] (ab92342) at 1/2000 dilution

      Lane 1 : Human fetal kidney tissue lysate
      Lane 2 : Human fetal brain tissue lysate
      Lane 3 : Human placenta tissue lysate
      Lane 4 : Human testis tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 74 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    • ab92342 at 1/250 dilution staining GTPBP4 in Human kidney by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.

    References for Anti-GTPBP4 antibody [EPR3500] (ab92342)

    ab92342 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab92342.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"