Anti-OLA1 antibody (ab229112)
Key features and details
- Rabbit polyclonal to OLA1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-OLA1 antibody
See all OLA1 primary antibodies -
Description
Rabbit polyclonal to OLA1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Rhesus monkey -
Immunogen
Recombinant fragment within Human OLA1 (internal sequence). The exact sequence is proprietary.
Database link: Q9NTK5 -
Positive control
- WB: HepG2, HEK-293T, HeLa and A431 whole cell lysates; Mouse brain lysate. IHC-P: Human colon carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 45 kDa. |
Target
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Function
Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP. -
Sequence similarities
Belongs to the GTP1/OBG family.
Contains 1 G (guanine nucleotide-binding) domain. - Information by UniProt
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Database links
- Entrez Gene: 424144 Chicken
- Entrez Gene: 509966 Cow
- Entrez Gene: 29789 Human
- Entrez Gene: 67059 Mouse
- Entrez Gene: 296488 Rat
- Entrez Gene: 697309 Rhesus monkey
- Omim: 611175 Human
- SwissProt: Q2HJ33 Cow
see all -
Alternative names
- DKFZp313H1942 antibody
- DNA damage-regulated overexpressed in cancer 45 protein antibody
- DOC45 antibody
see all
Images
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All lanes : Anti-OLA1 antibody (ab229112) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 45 kDa -
Anti-OLA1 antibody (ab229112) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 45 kDa10% SDS-PAGE gel.
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Anti-OLA1 antibody (ab229112) at 1/1000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 45 kDa10% SDS-PAGE gel.
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Paraffin-embedded human colon carcinoma tissue stained for OLA1 with ab229112 at 1/250 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab229112 has not yet been referenced specifically in any publications.