Overview

  • Product name
    Anti-GTPBP9 antibody
  • Description
    Rabbit polyclonal to GTPBP9
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: QGLGNAFLSH ISACDGIFHL TRAFEDDDIT HVEGSVDPIR DIEIIHEELQ, corresponding to amino acids 104-153 of Human GTPBP9

  • Positive control
    • Jurkat cell lysate, Human intestine This antibody gave a positive result in IF in the following Formaldehyde/Methanol fixed cell line: HCT116

Properties

Applications

Our Abpromise guarantee covers the use of ab51077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

Target

Images

  • Lane 1 : Marker
    Lane 2 : Anti-GTPBP9 antibody (ab51077) at 0.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg/ml with skim milk/ PBS at 5 %

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 45 kDa
    Observed band size : 45 kDa
  • ab51077 at 4.0 µg/ml staining epithelial cells of the intestinal villus from human intestine; paraffin embedded; magnification 400x.
  • ab51077 stained HCT116 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab51077 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Martínez-Baeza E  et al. Metal mixture (As-Cd-Pb)-induced cell transformation is modulated by OLA1. Mutagenesis 31:463-73 (2016). Read more (PubMed: 26984302) »
  • Matsuzawa A  et al. The BRCA1/BARD1-Interacting Protein OLA1 Functions in Centrosome Regulation. Mol Cell N/A:N/A (2013). WB ; Human . Read more (PubMed: 24289923) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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