ab51077 stained HCT116 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab51077 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-GTPBP9 antibody (ab51077)
This product has been referenced in:
Matsuzawa A et al. The BRCA1/BARD1-Interacting Protein OLA1 Functions in Centrosome Regulation. Mol CellN/A:N/A (2013).
Read more (PubMed: 24289923) »
Zhang J et al. OLA1, an Obg-like ATPase, suppresses antioxidant response via nontranscriptional mechanisms. Proc Natl Acad Sci U S A106:15356-61 (2009).
Read more (PubMed: 19706404) »