Overview

  • Product name
  • Description
    Rabbit polyclonal to GTSE1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: IDLMTNTPDM NKNVAKPSPV VGQLIDLSSP LIQLSPEADK ENVDSPLLKF , corresponding to a region within internal sequence amino acids 690 - 739 of Human GTSE1 (NP_057510)

  • Positive control
    • MCF7 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 77 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    GTSE1 is only expressed in the S and G2 phases of the cell cycle, where it co-localizes with cytoplasmic tubulin and microtubules. In response to DNA damage, GTSE1 accumulates in the nucleus and binds the tumor suppressor protein p53, shuttling it out of the nucleus and repressing its ability to induce apoptosis. Overexpression of GTSE1 delays G2/M phase progression.
  • Cellular localization
    Cytoplasm. Note: Associated with microtubules
  • Database links
  • Alternative names
    • B99 antibody
    • B99 homolog antibody
    • G 2 and S phase expressed 1 antibody
    • G two S phase expressed protein 1 antibody
    • GTSE 1 antibody
    see all

Images

  • Anti-GTSE1 antibody (ab83316) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 77 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

References

ab83316 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab83316.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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