Overview

  • Product name
    Anti-Guanylate Cyclase antibody
    See all Guanylate Cyclase primary antibodies
  • Description
    Rabbit polyclonal to Guanylate Cyclase
  • Specificity
    This antibody detects endogenous levels of total Guanylate Cyclase ß protein.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from Human Guanylate Cyclase ß

  • Positive control
    • HuvEc cell extract; Paraffin-embedded human lung carcinoma tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
ELISA 1/5000.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    Guanylate Cyclase is an heterodimer of an alpha and a beta chain. Its enzymatic activity transforms GTP to GMP releasing a diphosphate. It binds 1 or 2 hemes per heterodimer. It is activated by nitric oxide in the presence of magnesium or manganese ions. There are two types of guanylate cyclase: soluble form and membrane-associated receptor form.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • GC SB2 antibody
    • GCS beta 2 antibody
    • Guanylate cyclase 1 soluble beta 2 antibody
    • GUCY1B2 antibody

Images

  • All lanes : Anti-Guanylate Cyclase antibody (ab53084) at 1/300 dilution

    Lane 1 : HuvEc cell extract
    Lane 2 : HuvEc cell extract with peptide


    Predicted band size : 70 kDa
    Observed band size : 70 kDa
  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Guanylate Cyclase β antibody, ab53084, at 1/50 dilution.

References

This product has been referenced in:
  • Wu RY & Ma N Expression of nitric oxide synthase and guanylate cyclase in the human ciliary body and trabecular meshwork. Chin Med J (Engl) 125:129-33 (2012). WB, IHC-P ; Human . Read more (PubMed: 22340479) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab53084.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up