Anti-Guanylate Cyclase antibody [GC25] (ab20042)

Overview

  • Product name
    Anti-Guanylate Cyclase antibody [GC25]
    See all Guanylate Cyclase primary antibodies
  • Description
    Mouse monoclonal [GC25] to Guanylate Cyclase
  • Specificity
    Ab20042 recognizes the beta subunit of Human Soluble Guanylate Cyclase.
  • Tested applications
    Suitable for: Flow Cyt, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein: Recombinant human soluble guanylate cyclase alpha1 beta1 heterodimer.

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Ab20042 is purified from ascites.
  • Clonality
    Monoclonal
  • Clone number
    GC25
  • Myeloma
    Sp2/0
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 70 kDa.

Target

  • Relevance
    Guanylate Cyclase is an heterodimer of an alpha and a beta chain. Its enzymatic activity transforms GTP to GMP releasing a diphosphate. It binds 1 or 2 hemes per heterodimer. It is activated by nitric oxide in the presence of magnesium or manganese ions. There are two types of guanylate cyclase: soluble form and membrane-associated receptor form.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • GC SB2 antibody
    • GCS beta 2 antibody
    • Guanylate cyclase 1 soluble beta 2 antibody
    • GUCY1B2 antibody

Images

  • Overlay histogram showing HeLa cells stained with ab20042 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab20042, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References

This product has been referenced in:
  • Bahls M  et al. Mother's exercise during pregnancy programmes vasomotor function in adult offspring. Exp Physiol 99:205-19 (2014). Human . Read more (PubMed: 24163423) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Sample
Rat Tissue sections (retina)
Specification
retina
Permeabilization
Yes - Triton X-100 0.3%
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 03 2014

The originator of this antibody used a purified human soluble guanylate cyclase as a positive control antigen in Western blot testing. Unfortunately, this antigen is not available for sale. If you have any additional questions, please contact us ag...

Read More

Thank you for your enquiry. I would suggest a beta actin loading control (such as ab8227) or GAPDH (such as ab9485). If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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