Anti-Guanylyl Cyclase alpha 1 antibody (ab50358)

Overview

  • Product nameAnti-Guanylyl Cyclase alpha 1 antibody
    See all Guanylyl Cyclase alpha 1 primary antibodies
  • Description
    Rabbit polyclonal to Guanylyl Cyclase alpha 1
  • Specificityab50358 specifically recognizes Guanylyl Cyclase alpha 1.
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Cow, Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide:

    KDAEDGNANFLGKASGVD

    conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 673-690 of Rat Guanylyl Cyclase alpha 1

  • Positive control
    • Rat brain cytosolic fraction and trypsin digested, paraffin-embedded human, bovine and mouse heart sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab50358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
IHC-P
  • Application notesIHC-P: 1/100.
    IP: 5-10µg for 60-120µg of cytosolic fraction of rat brain
    WB: 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 83 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    References for Anti-Guanylyl Cyclase alpha 1 antibody (ab50358)

    This product has been referenced in:
    • Liu Y  et al. Volume overload induces differential spatiotemporal regulation of myocardial soluble guanylyl cyclase in eccentric hypertrophy and heart failure. J Mol Cell Cardiol 60:72-83 (2013). WB ; Dog . Read more (PubMed: 23567617) »
    • Leiria LO  et al. The Soluble Guanylyl Cyclase Activator BAY 60-2770 Ameliorates Overactive Bladder in Obese Mice. J Urol N/A:N/A (2013). Read more (PubMed: 24050894) »

    See all 6 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (Hela, Hep G2)
    Specification Hela, Hep G2
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 05 2015

    Thank you for your enquiry regarding ab50358 and ab84955 and for taking the time to provide some useful details of the experiments.

    I can confirm that these two antibodies are from two different sources and the immunogen sequences are also dif...

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    I am sorry to hear that you have been experiencing problems using these two products in the application that you wish.

    In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the applicati...

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    DISCOUNT CODE: ***********
    Expiration date: ************
    I am very pleased to hear you would like to accept our offer and test ab50358 in IIHC-Fr. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this off...

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    Thank you very much for your interest in ab50358.
    To our knowledge, ab50358 has not been tested in IHC-Fr. Therefore, I can offer a discount off a future purchase if you buy ab50358 now, test it in IHC-Fr and submit feedback to us in the form of a...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Sheep Tissue lysate - whole (Sheep lung tissue)
    Loading amount 40 µg
    Specification Sheep lung tissue
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 01 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"