Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferAntibody stabilization buffer, with preservatives, consisting of Hepes, NaCl, KCl, glycerol, BSA and chaotropic agents.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab42108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
WB
IHC (PFA fixed)
  • Application notesIHC-Fr: Use at an assay dependent dilution.
    Immunohistochemistry (PFA fixed): Use at an assay dependent dilution.
    WB: 1/500. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionHas guanylyl cyclase on binding to the beta-1 subunit.
      Isoform 2 acts as a negative regulator of guanylyl cyclase activity as it forms non-functional heterodimers with the beta subunits.
    • Tissue specificityIsoform 1 is expressed in fetal brain, liver, colon, endothelium and testis. Isoform 2 is expressed only in liver, colon and endothelium.
    • Sequence similaritiesBelongs to the adenylyl cyclase class-4/guanylyl cyclase family.
      Contains 1 guanylate cyclase domain.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • GC SA2 antibody
      • GCS alpha 2 antibody
      • GCS-alpha-2 antibody
      • GCYA2_HUMAN antibody
      • Guanylate cyclase soluble subunit alpha-2 antibody
      • GUC1A2 antibody
      • GUCSA2 antibody
      • GUCY1A2 antibody
      see all

    Anti-Guanylyl Cyclase alpha 2 antibody images

    • Anti-Guanylyl Cyclase alpha 2 antibody (ab42108) at 1/750 dilution + PC-soluble guanylate cyclase alfa 2

      Predicted band size : 98 kDa
      Observed band size : 98 kDa
    • Immunodetection of guanylyl cyclase alpha 2 in mouse brain tissue section using ab42108 (1/100). Please refer to abreview for further experimental details.

      See Abreview

    References for Anti-Guanylyl Cyclase alpha 2 antibody (ab42108)

    This product has been referenced in:
    • Buys ES  et al. sGC(alpha)1(beta)1 attenuates cardiac dysfunction and mortality in murine inflammatory shock models. Am J Physiol Heart Circ Physiol 297:H654-63 (2009). WB ; Mouse . Read more (PubMed: 19502556) »
    • De Backer O  et al. Role of the soluble guanylyl cyclase alpha1/alpha2 subunits in the relaxant effect of CO and CORM-2 in murine gastric fundus. Naunyn Schmiedebergs Arch Pharmacol 378:493-502 (2008). WB ; Mouse . Read more (PubMed: 18563392) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (brain)
    Specification brain
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Username

    Ute Neubacher

    Verified customer

    Submitted Feb 17 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"