Anti-Guanylyl Cyclase beta 1 antibody (ab24824)

Overview

  • Product nameAnti-Guanylyl Cyclase beta 1 antibody
    See all Guanylyl Cyclase beta 1 primary antibodies
  • Description
    Rabbit polyclonal to Guanylyl Cyclase beta 1
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Dog, Pig
  • Immunogen

    Synthetic peptide:

    EDFYEDLDRFEENGTQESR

    , corresponding to amino acids 189-207 of Human Guanylyl Cyclase beta 1

  • Positive control
    • Brain

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

Anti-Guanylyl Cyclase beta 1 antibody images

  • Human cerebellum stained with Anti Guanylyl cyclase beta 1 ab24824 at 1/100 dilution for 30 min at RT.
  • ICC/IF image of ab24824 stained SKNSH cells. The cells were Both and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24824, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Guanylyl Cyclase beta 1 antibody (ab24824)

This product has been referenced in:
  • Tsihlis ND  et al. Nitric oxide is less effective at inhibiting neointimal hyperplasia in spontaneously hypertensive rats. Nitric Oxide 35:165-74 (2013). IHC-P ; Rat . Read more (PubMed: 24149190) »
  • van Heerebeek L  et al. Low myocardial protein kinase g activity in heart failure with preserved ejection fraction. Circulation 126:830-9 (2012). IHC-P ; Human . Read more (PubMed: 22806632) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Right Heart Atria)
Loading amount 20 µg
Specification Right Heart Atria
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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Submitted Oct 22 2012

DISCOUNT CODE: 100%ABR-0000
Ablaufdatum: 27. Januar 2013

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"