Anti-GW182 antibody [4B6] - P/GW Body Marker (ab70522)

Overview

  • Product name
    Anti-GW182 antibody [4B6] - P/GW Body Marker
    See all GW182 primary antibodies
  • Description
    Mouse monoclonal [4B6] to GW182 - P/GW Body Marker
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein minus the amino terminus TNR domain.

  • Positive control
    • HEp-2000 cells
  • General notes

    This antibody clone is manufactured by Abcam.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Some batches contain 0.4M arginine. Please contact Scientific Support for further information.
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    4B6
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

Target

  • Function
    Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the GW182 family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localization
    Cytoplasm > P-body. Mammalian P-bodies are also known as GW bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAG repeat protein 26 antibody
    • CAGH26 antibody
    • DKFZp666E117 antibody
    • EDIE antibody
    • EMSY interactor protein antibody
    • FLJ22043 antibody
    • Glycine tryptophan protein of 182 kDa antibody
    • Glycine-tryptophan protein of 182 kDa antibody
    • GW1 antibody
    • GW182 antibody
    • GW182 autoantigen antibody
    • Hypothetical protein DKFZp566M143 antibody
    • KIAA1460 antibody
    • MGC75384 antibody
    • OTTHUMP00000122485 antibody
    • OTTHUMP00000195164 antibody
    • OTTHUMP00000195165 antibody
    • Protein GW1 antibody
    • TNR6A_HUMAN antibody
    • TNRC 6A antibody
    • TNRC6 antibody
    • Tnrc6a antibody
    • Trinucleotide repeat containing 6A antibody
    • Trinucleotide repeat containing gene 6A protein antibody
    • Trinucleotide repeat-containing gene 6A protein antibody
    see all

Images

  • ab70522 staining GW182 in pre-fixed HEp-2000 cells. ab70522 was used at 1/100 dilution and incubated overnight at 4°C. An Alexa Fluor® 488-conjugated anti-mouse IgG was used as the secondary antibody.

  • ab70522 (1/200) staining GW182 in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

    See Abreview

References

This product has been referenced in:
  • Coppin L  et al. Galectin-3 is a non-classic RNA binding protein that stabilizes the mucin MUC4 mRNA in the cytoplasm of cancer cells. Sci Rep 7:43927 (2017). Read more (PubMed: 28262838) »
  • Douglas JN  et al. Antibodies to the RNA Binding Protein Heterogeneous Nuclear Ribonucleoprotein A1 Colocalize to Stress Granules Resulting in Altered RNA and Protein Levels in a Model of Neurodegeneration in Multiple Sclerosis. J Clin Cell Immunol 7:402 (2016). Read more (PubMed: 27375925) »

See all 10 Publications for this product

Customer reviews and Q&As


Unfortunately, I have been informed that the ab70522 Anti-GW182 antibody [4B6] - P/GW Body Marker has not been tested in Xenopus laevis. This was an error and our datasheet has now been updated so that Xenopus laevis is no longer listed as a tes...

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DISCOUNT CODE:***
Expiration date: January 24, 2013

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forAb87541 in ICC/IFand include this code in the “Addit...

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DISCOUNT CODE: [XXXXX] Expiration date: [07/03/2012] I am very pleased to hear you would like to accept our offer and test ab[87541] in [ICC/IF]. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this off...

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Thank you for contacting us. We have 3 antibodies available in catalogue ab114857, ab87541 and ab84403. These antibodies are Rabbit polyclonal to GW182. Could you check the datasheets and select the product which is suitable for you. All of the...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk Mcmanus

Verified customer

Submitted May 22 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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