Anti-GW182 antibody [4B6] - P/GW Body Marker (ab70522)


  • Product nameAnti-GW182 antibody [4B6] - P/GW Body Marker
    See all GW182 primary antibodies
  • Description
    Mouse monoclonal [4B6] to GW182 - P/GW Body Marker
  • Tested applicationsSuitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein minus the amino terminus TNR domain.

  • Positive control
    • HEp-2000 cells



Our Abpromise guarantee covers the use of ab70522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.


  • FunctionPlays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the GW182 family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localizationCytoplasm > P-body. Mammalian P-bodies are also known as GW bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAG repeat protein 26 antibody
    • CAGH26 antibody
    • DKFZp666E117 antibody
    • EDIE antibody
    • EMSY interactor protein antibody
    • FLJ22043 antibody
    • Glycine tryptophan protein of 182 kDa antibody
    • Glycine-tryptophan protein of 182 kDa antibody
    • GW1 antibody
    • GW182 antibody
    • GW182 autoantigen antibody
    • Hypothetical protein DKFZp566M143 antibody
    • KIAA1460 antibody
    • MGC75384 antibody
    • OTTHUMP00000122485 antibody
    • OTTHUMP00000195164 antibody
    • OTTHUMP00000195165 antibody
    • Protein GW1 antibody
    • TNR6A_HUMAN antibody
    • TNRC 6A antibody
    • TNRC6 antibody
    • Tnrc6a antibody
    • Trinucleotide repeat containing 6A antibody
    • Trinucleotide repeat containing gene 6A protein antibody
    • Trinucleotide repeat-containing gene 6A protein antibody
    see all

Anti-GW182 antibody [4B6] - P/GW Body Marker images

  • ab70522 staining GW182 in pre-fixed HEp-2000 cells. ab70522 was used at 1/100 dilution and incubated overnight at 4°C. An Alexa Fluor® 488-conjugated anti-mouse IgG was used as the secondary antibody.

  • ab70522 (1/200) staining GW182 in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

    See Abreview

References for Anti-GW182 antibody [4B6] - P/GW Body Marker (ab70522)

This product has been referenced in:
  • Li S  et al. TRIM65 regulates microRNA activity by ubiquitination of TNRC6. Proc Natl Acad Sci U S A 111:6970-5 (2014). Read more (PubMed: 24778252) »
  • Moser JJ & Fritzler MJ Cytoplasmic ribonucleoprotein (RNP) bodies and their relationship to GW/P bodies. Int J Biochem Cell Biol 42:828-43 (2010). Read more (PubMed: 19944184) »

See all 7 Publications for this product

Product Wall

Unfortunately, I have been informed that the ab70522 Anti-GW182 antibody [4B6] - P/GW Body Marker has not been tested in Xenopus laevis. This was an error and our datasheet has now been updated so that Xenopus laevis is no longer listed as a tes...

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Expiration date: January 24, 2013

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forAb87541 in ICC/IFand include this code in the “Addit...

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DISCOUNT CODE: [XXXXX] Expiration date: [07/03/2012] I am very pleased to hear you would like to accept our offer and test ab[87541] in [ICC/IF]. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this off...

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Thank you for contacting us. We have 3 antibodies available in catalogue ab114857, ab87541 and ab84403. These antibodies are Rabbit polyclonal to GW182. Could you check the datasheets and select the product which is suitable for you. All of the...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS

Dr. Kirk McManus

Verified customer

Submitted May 22 2009