Overview

  • Product nameAnti-GW182 antibody
    See all GW182 primary antibodies
  • Description
    Rabbit polyclonal to GW182
  • Tested applicationsSuitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1100 - 1200 of Human GW182.

  • Positive control
    • This antibody gave a positive signal in HeLa cell line.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab110233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionPlays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the GW182 family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localizationCytoplasm > P-body. Mammalian P-bodies are also known as GW bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAG repeat protein 26 antibody
    • CAGH26 antibody
    • DKFZp666E117 antibody
    • EDIE antibody
    • EMSY interactor protein antibody
    • FLJ22043 antibody
    • Glycine tryptophan protein of 182 kDa antibody
    • Glycine-tryptophan protein of 182 kDa antibody
    • GW1 antibody
    • GW182 antibody
    • GW182 autoantigen antibody
    • Hypothetical protein DKFZp566M143 antibody
    • KIAA1460 antibody
    • MGC75384 antibody
    • OTTHUMP00000122485 antibody
    • OTTHUMP00000195164 antibody
    • OTTHUMP00000195165 antibody
    • Protein GW1 antibody
    • TNR6A_HUMAN antibody
    • TNRC 6A antibody
    • TNRC6 antibody
    • Tnrc6a antibody
    • Trinucleotide repeat containing 6A antibody
    • Trinucleotide repeat containing gene 6A protein antibody
    • Trinucleotide repeat-containing gene 6A protein antibody
    see all

Anti-GW182 antibody images

  • ICC/IF image of ab110233 stained HeLa cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110233, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% paraformaldehyde fixed (10 min) MCF7 cells at 1µg/ml.

References for Anti-GW182 antibody (ab110233)

ab110233 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.05% Triton X100 in PBS
Username

Dr. Kirk McManus

Verified customer

Submitted May 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"