Overview

  • Product name
  • Description
    Rabbit polyclonal to GW182
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Macaque monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human GW182.

  • Positive control
    • This antibody gave a positive signal in MCF7 cell line within ICC/IF as well as in NIH3T3 within WB.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab156173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 270 kDa (predicted molecular weight: 210 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the GW182 family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localization
    Cytoplasm > P-body. Mammalian P-bodies are also known as GW bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAG repeat protein 26 antibody
    • CAGH26 antibody
    • DKFZp666E117 antibody
    • EDIE antibody
    • EMSY interactor protein antibody
    • FLJ22043 antibody
    • Glycine tryptophan protein of 182 kDa antibody
    • Glycine-tryptophan protein of 182 kDa antibody
    • GW1 antibody
    • GW182 antibody
    • GW182 autoantigen antibody
    • Hypothetical protein DKFZp566M143 antibody
    • KIAA1460 antibody
    • MGC75384 antibody
    • OTTHUMP00000122485 antibody
    • OTTHUMP00000195164 antibody
    • OTTHUMP00000195165 antibody
    • Protein GW1 antibody
    • TNR6A_HUMAN antibody
    • TNRC 6A antibody
    • TNRC6 antibody
    • Tnrc6a antibody
    • Trinucleotide repeat containing 6A antibody
    • Trinucleotide repeat containing gene 6A protein antibody
    • Trinucleotide repeat-containing gene 6A protein antibody
    see all

Images

  • ICC/IF image of ab156173 stained MCF7 cells. The cells were 100% methanol fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab156173, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa and HepG2 cells at 5µg/ml.
  • Anti-GW182 antibody (ab156173) at 1 µg/ml + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 210 kDa
    Observed band size: 260 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 31 kDa, 50 kDa, 60 kDa, 75 kDa, 98 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes


    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab156173 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab156173 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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