Recombinant
RabMAb

Anti-GYPB antibody [EPR15454(B)] (ab190351)

Overview

  • Product name
    Anti-GYPB antibody [EPR15454(B)]
    See all GYPB primary antibodies
  • Description
    Rabbit monoclonal [EPR15454(B)] to GYPB
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human GYPB aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P06028

  • Positive control
    • Human fetal liver, K562, mouse brain, kidney and spleen, rat brain, C6, Raw264.7, PC-12, and NIH/3T3 lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR15454(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 15 kDa (predicted molecular weight: 10 kDa).

Target

  • Function
    This protein is a minor sialoglycoprotein in erythrocyte membranes.
  • Sequence similarities
    Belongs to the glycophorin-A family.
  • Post-translational
    modifications
    The N-terminal extracellular domain is heavily glycosylated on serine and threonine residues.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD235b antibody
    • GLPB_HUMAN antibody
    • Glycophorin-B antibody
    • GYPB antibody
    • PAS-3 antibody
    • Sialoglycoprotein delta antibody
    • SS-active sialoglycoprotein antibody
    see all

Images

  • All lanes : Anti-GYPB antibody [EPR15454(B)] (ab190351) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat brain lysate
    Lane 5 : C6 lysate
    Lane 6 : Raw264.7 lysate
    Lane 7 : PC12 lysate
    Lane 8 : NIH/3T3 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 10 kDa
    Observed band size: 15 kDa (why is the actual band size different from the predicted?)

  • Anti-GYPB antibody [EPR15454(B)] (ab190351) at 1/10000 dilution + K562 lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 10 kDa
    Observed band size: 15 kDa (why is the actual band size different from the predicted?)

  • Anti-GYPB antibody [EPR15454(B)] (ab190351) at 1/1000 dilution + Human fetal liver at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 10 kDa
    Observed band size: 15 kDa (why is the actual band size different from the predicted?)

References

ab190351 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab190351.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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