The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 29 kDa).
RelevanceEAF1 belongs to the EAF family. It acts as a transcriptional transactivator of ELL and ELL2 elongation activities. It is strongly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine and colon; poorly expressed in thymus.
Cellular localizationNucleus speckle. Nucleus, Cajal body.
Note: Colocalizes with ELL to nuclear speckles. Also localized to Cajal (coiled) bodies.