Overview

  • Product nameAnti-H00085403-B01 antibody
  • Description
    Mouse polyclonal to H00085403-B01
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length EAF1 protein (Human)

  • Positive control
    • EAF1 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 29 kDa).

Target

  • RelevanceEAF1 belongs to the EAF family. It acts as a transcriptional transactivator of ELL and ELL2 elongation activities. It is strongly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine and colon; poorly expressed in thymus.
  • Cellular localizationNucleus speckle. Nucleus, Cajal body. Note: Colocalizes with ELL to nuclear speckles. Also localized to Cajal (coiled) bodies.
  • Database links
  • Alternative names
    • ELL (eleven nineteen lysine rich leukemia gene) associated factor 1 antibody

Anti-H00085403-B01 antibody images

  • All lanes : Anti-H00085403-B01 antibody (ab68255) at 1/500 dilution

    Lane 1 : EAF1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 29 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References for Anti-H00085403-B01 antibody (ab68255)

ab68255 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"