H2A.X (pSer139) Human In-Cell ELISA Kit (IR) (ab131382)
- Product nameH2A.X (pSer139) Human In-Cell ELISA Kit (IR)
- Detection methodIR
- Tests1 x 96 well plate
- Sample typeAdherent cells, Suspension cells
- Assay typeCell-based (qualitative)
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
The H2A.X phospho Ser139 Human In-Cell ELISA Kit (IR) (ab131382) is designed to study the induction of DNA damage in response to various stimuli. A rabbit monoclonal antibody specific to H2A.X phospho Ser139 is used in this high-throughput duplexing plate-based assay. H2A.X phospho Ser139 is a reliable readout for double-stranded DNA breaks.
Plates are available in our ICE (In-Cell ELISA) Support Pack (ab111542) which can be bought seperately.
- Tested applicationsIn-Cell ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 1000X IRDye®-Labeled Secondary Antibody 1 x 24µl 100X Anti-H2A.X (pSer139) Rabbit Primary Antibody 1 x 120µl 100X Triton X-100 1 x 0.5ml 10X Blocking Buffer 1 x 10ml 10X Phosphate Buffered Saline 1 x 100ml 400X Tween-20 1 x 2ml Janus Green Stain 1 x 17ml
- Research Areas
- SwissProt: P16104 Human
Our Abpromise guarantee covers the use of ab131382 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|In-Cell ELISA||In-Cell ELISA|
H2A.X (pSer139) Human In-Cell ELISA Kit (IR) images
Sample experiment using ab131382 on HeLa cells following drug treatment: H2A.X (pSer139) readout. HeLa cells were treated for 4 hours with dose titrations of Camptothecin, Etoposide and Staurosporin. The dashed grey line indicates the vehicle control signal.
Specificity of H2A.X (pSer139) antibodies demonstrated by immunocytochemistry. The primary antibody used in this assay kit was validated by staining HeLa cells treated with 10 µM Camptothecin or vehicle for 4 hours and imaged by fluorescent microscopy. Note the absence of H2A.X (pSer139) in the untreated cells.
All lanes : H2A.X (pSer139) Human In-Cell ELISA Kit (IR) (ab131382)
Lane 1 : Untreated cells
Lane 2 : Camptothecin treated cells
Lane 3 : UV exposed cells
Antibody specificity demonstrated by Western Blot Analysis: H2A.X (pSer139) is phospho-specific. Jurkat cells were stimulated with UV light exposure to induce H2A.X phospho Ser139 and then the UV treated lysate was treated with lambda protein phosphatase. Top panel: H2A.X (pSer139) is induced by UV treatment and the western blot band is sensitive to phosphatase treatment. Lower panel: In contrast, total H2A.X (ab124781) levels are not sensitive to phosphatase treatment.
References for H2A.X (pSer139) Human In-Cell ELISA Kit (IR) (ab131382)
ab131382 has not yet been referenced specifically in any publications.