MitoSciences (MS1099)

H2A.X (pSer139) Human In-Cell ELISA Kit (IR) (ab131382)

Overview

  • Product nameH2A.X (pSer139) Human In-Cell ELISA Kit (IR)
  • Detection methodIR
  • Tests
    1 x 96 well plate
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeCell-based (qualitative)
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    The H2A.X phospho Ser139 Human In-Cell ELISA Kit (IR) (ab131382) is designed to study the induction of DNA damage in response to various stimuli. A rabbit monoclonal antibody specific to H2A.X phospho Ser139 is used in this high-throughput duplexing plate-based assay. H2A.X phospho Ser139 is a reliable readout for double-stranded DNA breaks.

     

    Plates are available in our ICE (In-Cell ELISA) Support Pack (ab111542) which can be bought seperately.

  • Tested applicationsIn-Cell ELISA more details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab131382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
In-Cell ELISA Use at an assay dependent concentration.

H2A.X (pSer139) Human In-Cell ELISA Kit (IR) images

  • Sample experiment using ab131382 on HeLa cells following drug treatment: H2A.X (pSer139) readout. HeLa cells were treated for 4 hours with dose titrations of Camptothecin, Etoposide and Staurosporin. The dashed grey line indicates the vehicle control signal.
  • Specificity of H2A.X (pSer139) antibodies demonstrated by immunocytochemistry. The primary antibody used in this assay kit was validated by staining HeLa cells treated with 10 µM Camptothecin or vehicle for 4 hours and imaged by fluorescent microscopy. Note the absence of H2A.X (pSer139) in the untreated cells.
  • All lanes : H2A.X (pSer139) Human In-Cell ELISA Kit (IR) (ab131382)

    Lane 1 : Untreated cells
    Lane 2 : Camptothecin treated cells
    Lane 3 : UV exposed cells
  • Antibody specificity demonstrated by Western Blot Analysis: H2A.X (pSer139) is phospho-specific. Jurkat cells were stimulated with UV light exposure to induce H2A.X phospho Ser139 and then the UV treated lysate was treated with lambda protein phosphatase. Top panel: H2A.X (pSer139) is induced by UV treatment and the western blot band is sensitive to phosphatase treatment. Lower panel: In contrast, total H2A.X (ab124781) levels are not sensitive to phosphatase treatment.

Protocols

References for H2A.X (pSer139) Human In-Cell ELISA Kit (IR) (ab131382)

ab131382 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"