Anti-H2A.Z (acetyl K3 + K8 + K10 + K14) antibody (ab43983)

Overview

  • Product nameAnti-H2A.Z (acetyl K3 + K8 + K10 + K14) antibody
    See all H2A.Z primary antibodies
  • Description
    Sheep polyclonal to H2A.Z (acetyl K3 + K8 + K10 + K14)
  • Specificityab43983 was raised against an acetylated N-terminal H2A.Z peptide at residues K3, K8, K10 and K14. However, it is currently unclear if the antibody recognises all of these modifications or indeed unmodified H2A.Z. Customer feedback on this matter is appreciated.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
    Does not react with: Chicken, Cow
  • Immunogen

    Synthetic peptide corresponding to Saccharomyces cerevisiae H2A.Z aa 1-18 (N terminal) (acetyl K3 + K8 + K10 + K14).
    Sequence:

    SGK(Ac)AHGGK(Ac)GK(Ac)SGAK(Ac)DSGS-C


    Database link: P0C0S5

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab43983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).

Target

  • FunctionVariant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
  • Sequence similaritiesBelongs to the histone H2A family.
  • Post-translational
    modifications
    Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
    Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
    Not phosphorylated.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt
  • Alternative names
    • H2A/z antibody
    • H2afz antibody
    • H2AZ antibody
    • H2AZ_HUMAN antibody
    • Histone H2A.Z antibody
    see all

Anti-H2A.Z (acetyl K3 + K8 + K10 + K14) antibody images

  • Anti-H2A.Z (acetyl K3 + K8 + K10 + K14) antibody (ab43983) at 2 µg/ml + S. cerevisiae lysate at 2 µg/ml

    Predicted band size : 14 kDa

References for Anti-H2A.Z (acetyl K3 + K8 + K10 + K14) antibody (ab43983)

ab43983 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us and for your interest in our products.

Unfortunately we have yet to receieve the Abreview concerning ChIP for ab43983. All tested applications are specified on our datasheets, and these are updated as soon as any n...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"