The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Oxidizes glucose-6-phosphate and glucose, as well as other hexose-6-phosphates.
Present in most tissues examined, strongest in liver.
Involvement in disease
Defects in H6PD are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).
In the N-terminal section; belongs to the glucose-6-phosphate dehydrogenase family. In the C-terminal section; belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling H6PD with ab84353 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of alveolar type I cells. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - H6PD. Right - Merge.
Western blot - H6PD antibody (ab84353)
Anti-H6PD antibody (ab84353) at 1 µg/ml + 721_B cell lysate at 10 µg
Secondary anti-Rabbit IgG HRP at 1/50000 dilution
Predicted band size : 89 kDa Observed band size : 89 kDa Additional bands at : 42 kDa,65 kDa. We are unsure as to the identity of these extra bands.