Overview

  • Product nameAnti-hCAP-D3 antibodySee all hCAP-D3 primary antibodies ...
  • Description
    Rabbit polyclonal to hCAP-D3
  • SpecificityWe have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsWB, IP more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between C terminal residues 1450-1507 of human hCAP-D3. (NP_056076.1)

  • Positive control
    • HeLa and 293T whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab70349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IP
  • Application notesIP: Use at 1 to 4 µg/mg of lysate
    WB: 1/2000 - 1/10000. Detects a band of approximately 174 kDa (predicted molecular weight: 168 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRegulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis.
    • Sequence similaritiesContains 4 HEAT repeats.
    • Cellular localizationNucleus.
    • Target information above from: UniProt accession P42695 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • CAP D3 antibody
      • CAPD 3 antibody
      • CAPD3 antibody
      • CNDD3_HUMAN antibody
      • Condensin II complex subunit D3 antibody
      • Condensin-2 complex subunit D3 antibody
      • FLJ42888 antibody
      • hCAP-D3 antibody
      • Hcp 6 antibody
      • Hcp6 antibody
      • hHCP 6 antibody
      • hHCP6 antibody
      • KIAA0056 antibody
      • MGC104671 antibody
      • NCAPD 3 antibody
      • NCAPD3 antibody
      • Non SMC condensin II complex subunit D3 antibody
      • Non-SMC condensin II complex subunit D3 antibody
      • OTTHUMP00000235403 antibody
      • OTTHUMP00000235404 antibody
      see all

    Anti-hCAP-D3 antibody images

    • All lanes:200µg Hela whole cell lysate and ab70349 at 1µg/ml. Lane 1: ab70349 at 3 µg/mg lysate for IP. Lane 2: IgG control.
    • All lanes : Anti-hCAP-D3 antibody (ab70349) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg


      Predicted band size : 168 kDa
      Observed band size : 174 kDa (why is the actual band size different from the predicted?)

    References for Anti-hCAP-D3 antibody (ab70349)

    This product has been referenced in:
    • Liu W  et al. PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression. Nature 466:508-12 (2010). WB ; Human . Read more (PubMed: 20622854) »
    • Centore RC  et al. CRL4(Cdt2)-mediated destruction of the histone methyltransferase Set8 prevents premature chromatin compaction in S phase. Mol Cell 40:22-33 (2010). WB ; Human . Read more (PubMed: 20932472) »

    See all 2 Publications for this product

    Product Wall

    Abreviews
    Application Western blot
    Sample Mouse Cell lysate - whole cell (fibroblast)
    Loading amount 50 µg
    Specification fibroblast
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    Submitted May 10 2013

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