Overview

  • Product nameAnti-hCG antibody
    See all hCG primary antibodies
  • Description
    Rabbit polyclonal to hCG
  • SpecificityThis antibody reacts with hCG. It stains strongly with the human placenta and placental trophoblasts. No non-specific stainingwith any other tissue has been observed.
  • Tested applicationsIHC-Fr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Beta chain of human chorionic gonadotropin.

Properties

Applications

Our Abpromise guarantee covers the use of ab9376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
IHC-P
WB
  • Application notesIHC: This antibody may be diluted to a titer of 1:100-1:500 in an ABC method.
    Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
    Although not required, proteolytic treatment may enhance the immunostaining in overfixed tissues.
  • Target

    • RelevanceHuman chorionic gonadotropin (hCG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. Maintenance of the fetus in the first trimester of pregnancy requires the production of hCG, which binds to the corpus luteum of the ovary which is stimulated to produce progesterone which in turn maintains the secretory endometrium. hCG is present only in trace amounts in non pregnant urine and sera. It rises sharply during pregnancy. HCG is composed of two non identical, non covalently linked polypeptide chains designated as the a and b subunits. The a subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP) and HCG but negative for EMA and AFP is probably a choriocarcinoma.
    • Cellular localizationSecreted
    • Database links
    • Alternative names
      • Anterior pituitary glycoprotein hormones common subunit alpha antibody
      • CG ALPHA antibody
      • CG beta a antibody
      • CG beta antibody
      • CG-ALPHA antibody
      • CGA antibody
      • CGB antibody
      • CGB1 antibody
      • CGB2 antibody
      • CGB3 antibody
      • CGB5 antibody
      • CGB7 antibody
      • CGB8 antibody
      • Choriogonadotropin alpha chain antibody
      • Choriogonadotropin subunit beta antibody
      • Chorionic gonadotrophin alpha subunit antibody
      • Chorionic gonadotrophin chain beta antibody
      • chorionic gonadotrophin subunit alpha antibody
      • Chorionic gonadotropin alpha polypeptide antibody
      • Chorionic gonadotropin beta 3 subunit antibody
      • Chorionic gonadotropin beta 5 subunit antibody
      • Chorionic gonadotropin beta 7 subunit antibody
      • Chorionic gonadotropin beta 8 subunit antibody
      • Chorionic gonadotropin beta chain antibody
      • Chorionic gonadotropin beta polypeptide 1 antibody
      • Chorionic gonadotropin beta polypeptide 2 antibody
      • Chorionic gonadotropin beta polypeptide 5 antibody
      • Chorionic gonadotropin beta polypeptide 7 antibody
      • Chorionic gonadotropin beta polypeptide antibody
      • Chorionic gonadotropin beta subunit antibody
      • chorionic gonadotropin, alpha polypeptide antibody
      • chorionic gonadotropin, beta polypeptide 3 antibody
      • chorionic gonadotropin, beta polypeptide antibody
      • Follicle stimulating hormone alpha chain antibody
      • follicle-stimulating hormone alpha chain antibody
      • follicle-stimulating hormone alpha subunit antibody
      • Follitropin alpha chain antibody
      • FSH alpha antibody
      • FSH-alpha antibody
      • FSHA antibody
      • glycoprotein hormones alpha chain antibody
      • Glycoprotein hormones alpha chain precursor antibody
      • Glycoprotein hormones alpha polypeptide antibody
      • glycoprotein hormones, alpha polypeptide antibody
      • GPHA 1 antibody
      • GPHa antibody
      • GPHA1 antibody
      • HCG antibody
      • hCGB antibody
      • Human Chorionic Gonadotropin antibody
      • LHA antibody
      • LSH alpha antibody
      • LSH-alpha antibody
      • Luteinizing hormone alpha chain antibody
      • Lutropin alpha chain antibody
      • MGC119822 antibody
      • Thyroid stimulating hormone alpha chain antibody
      • thyroid-stimulating hormone alpha chain antibody
      • Thyrotropin alpha chain antibody
      • TSH alpha antibody
      • TSH-alpha antibody
      • TSHA antibody
      see all

    Anti-hCG antibody images

    • Human normal placenta. Staining is localised extracellular and in the cytoplasm. Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for rabbit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
    • ab9376 staining hCG in formalin-fixed, paraffin-embedded Human placenta by Immunohistochemistry.

    References for Anti-hCG antibody (ab9376)

    This product has been referenced in:
    • Wang JM  et al. The human leukocyte antigen G promotes trophoblast fusion and ß-hCG production through the Erk1/2 pathway in human choriocarcinoma cell lines. Biochem Biophys Res Commun 434:460-5 (2013). Read more (PubMed: 23583402) »
    • Dukic-Stefanovic S  et al. Chorionic gonadotropin and its receptor are both expressed in human retina, possible implications in normal and pathological conditions. PLoS One 7:e52567 (2012). IHC-Fr ; Human . Read more (PubMed: 23285091) »

    See all 3 Publications for this product

    Product Wall

    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (trophoblast)
    Loading amount 10 µg
    Specification trophoblast
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step 0.5%Casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Kai Wang

    Verified customer

    Submitted Apr 19 2012

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    I am very pleased to hear you would like to accept our offer and test ab9376 in western blot. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this...

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    Thank you for contacting Abcam.   The average Ig concentration for this antibody, ab9376 is 1.7g/L and the molecular weight is 40 kDa.   If there is anything else I can help you with, please let me know. .

    Dear Sir/Madam I am afraid that this antibody is not available in a form conjugated to a matrix. However, I believe Mobitec and Amersham Biosciences may be able to supply you with the matrices to conjugate our antibody to. I believe the procedure...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"